Objective To investigate the effect of high risk human papilloma virus (HPV) infection and typing on cervical lesions.MethodSelected 1480 cases of opportunistic cervical cancer screening in outpatient admissions of the 105th Hospital of Chinese People's Liberation Army from January 2013 to January 2016, collected the cervical exfoliated cells by real-time polymerase chain reaction (RT-PCR) method for genotype of 17 high risk HPV. The high risk HPV infection and the relationship between various types and cervical lesions were analyzed.Result A total of 268 patients had a complete and accurate pathologic examination in 282 cases of high risk HPV infection positive patients, 282 patients with high risk HPV were detected in all of the 1480 patients, the infection rate was 19.06%, among them, 40 cases (14.18%) were multiple infection, and 242 cases (85.82%) were single infection, the subtypes of the top three were HPV 52, HPV 16 and HPV 58. 38 cases (14.18%) of HPV 16, 68 cases (25.37%) of HPV 52, 62 cases (23.13%) of HPV 58 in the 268 patients diagnosed with cervical disease, the severity of the three subtypes were significantly different (P< 0.05). 40 cases (100.00%) patients of multiple infections were morbidity, 228 cases (94.21%) patients of single infection were morbidity, compared with patients with mild cervical lesions (CINⅠ and chronic cervicitis), the proportion of patients with severe cervical lesions (CINⅡ~Ⅲ and cervical cancer) was higher than that of patients with minor cervical lesions (P< 0.05).Conclusion High risk HPV infection and type are closely related to cervical lesions, The HPV 16, HPV 52 and HPV 58 have the highest detection rate among the type of high risk HPV. Most infection of cervical lesions are single infection, and patients with severe cervical lesions have higher incidence of multiple infections.%目的 探讨高危型人乳头瘤病毒(human papilloma virus,HPV)感染及分型对宫颈病变的影响.方法 选取本院门诊2013年1月至2016年1月接诊的机会性宫颈癌筛查病例1480例为研究对象,采集其宫颈脱落细胞,采用实时荧光聚合酶链反应(real-time polymerase chain reaction,RT-PCR)检测17种高危型HPV分型.分析高危型HPV感染及各分型与宫颈病变的关系.结果 282例高危型HPV感染阳性患者中,268例患者有完整准确的病理检查报告;1480例患者中,282例检出高危型HPV,感染率为19.06%,其中多重感染40例(14.18%),单一感染242例(85.82%),构成比前三位的亚型分别为HPV 52、HPV 16、HPV 58;268例确诊为宫颈疾病的患者中,HPV 16型38例(14.18%),HPV 52型68例(25.37%),HPV 58型62例(23.13%),3种亚型的病变程度比较均具有显著差异(P<0.05);282例高危型HPV阳性患者中多重感染40例(100.00%)发病,单一感染228例(94.21%)发病,宫颈病变较严重(CINⅡ~Ⅲ和宫颈癌)的患者多重感染比例高于轻微宫颈病变(CINⅠ和慢性宫颈炎)患者(P<0.05).结论 高危型HPV感染及分型与宫颈病变密切相关,其中HPV 16、HPV 52、HPV 58检出率最高,宫颈病变以单一感染为主,宫颈病变较严重的患者多重感染发生率较高.
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