Objective To detect the CpG island methylation status of p16, hMLH1 in sporadic colorectal cancer (SCRC), adenoma, normal colon tissues and evaluate the role and clinical significance of methylation of pi6, hMLH1 in colon cancer. Methods Colon tissues in Sichuan Provincial People's Hospital from October to December, 2008 were collected. The methylation status of p16, hMLH1 were analyzed by MSP, including 20 cases of SCRC, 20 cases of coIonic adenomas, 20 cases of normal tissues. Results The positive rate of p16 promoter methylation had statistically significant difference in SCRC compared with adenoma and normal tissues ( P = 0. 020, 0. 020), but there was no statistically significant difference between adenoma and normal tissues ( P ＞ 0.05 ). The postivive rate of hMLH1 promoter methylation had significant difference in SCRC compared with normal tissues ( P ＜ 0.05 ), but there was no significant difference in other groups (P ＞0.05). The methylation status of p16, hMHL1 were associated with Dukes stage, tumor size, lymph node metastasis and the degree of differentiation ( P ＜ 0.05 ), but not associated with tumor site and gender ( P ＞ 0.05 ). Conclusion Methylation of the promoter CpG islands of p16, hMLH1 is an important genetic event in colon cancer. Methylation of the promoter CpG islands of p16, hMLH1 may be associated with Dukes stage of colon cancer.%目的 检测正常黏膜、结肠腺瘤、结肠癌中p16、hMLH1基因CpG岛甲基化状态,评价其在结肠癌发生中的作用及临床意义.方法 收集四川省人民医院2008年10月～12月结肠组织标本60例,其中结肠癌 20例、腺瘤20例、正常组织标本20例.采用特异性甲基化PCR(MSP)方法检测p16、hMLH1基因的CpG岛甲基化状态.结果 MSP方法检测发现p16启动子CpG岛甲基化阳性表达率结肠癌组与腺瘤组、正常组比较有显著性差异(P=0.020、0.020),而腺瘤组与正常组比较无显著性差异(P>0.05).hMLH1启动子CpG岛甲基化阳性表达率结肠癌组与正常组比较有显著性差异(P=0.047),结肠癌组与腺瘤组、腺瘤组与正常组比较无显著性差异(P>0.05).p16、hMLH1基因CpG岛甲基化与Dukes分期、肿瘤大小、淋巴结转移、分化程度具有相关性(P<0.05),与肿瘤部位、性别无相关性(P>0.05).结论 p16、hMLH1启动子区CpG岛甲基化是促进结肠癌发生的重要基因事件,且可能与结肠癌Duke分期相关.
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