Objective To observe the effects of Smad-7 united Timp-1 siRNA expression plasmids on rat liver fibrosis. Methods The constructed Smad-7 and Timp-lsiRNA expression plasmids could be injected into rats abdominal cavity of composite factors duplicated liver fibrosis model by encapsulated with Lipofeetmine 2000. The pathological grade study was observed by HE staining, the expression of collagen type Ⅰ was assessed by Western blot, the mRNA expression of Smad-2 and Timp-1 were assessed by semi-RT-PCR. Results The pathological grade study in each treatment group and the normal group was significantly lower than that in the liver fibrosis group ( P ＜0.01 ). The expression of the collage type Ⅰ was lower obviously in each sole treatment group than that in the liver fibrosis group ( P ＜ 0.05 ),but the union treatment group was better (P ＜0.01 ). The mRNA expression of Smad-2 and Timp-1 in the union treatment group was lower than that in sole treatment group (P ＜ 0.05). Conclusion The curative effect of Smad-7 united Timp-1 siRNA plasmids on rat liver fibrosis is better than that of sole treatment.%目的观察Smad-7与Timp-1siRNA真核细胞表达质粒联合作用对大鼠肝纤维化的影响.方法 采用已构建的重组Smad-7与Timp-1siRNA真核细胞表达质粒,用脂质体包埋法通过腹腔注射将其导入肝纤维化模型大鼠体内.通过病理形态学、Western blot法及半定量RT-PCR法观察两种质粒联合作用对大鼠肝纤维化的影响.结果 两种质粒联合治疗组与各单一治疗组之间病理学分级有显著性差异(P<0.05);两种质粒联合治疗组中Ⅰ型胶原表达量显著低于单一治疗组(P<0.05),并较单一治疗组明显抑制Smad-2、Timp-1mRNA在肝纤维化肝脏中的表达(P<0.05).结论 Smad-7与Timp-1siRNA表达质粒联合抑制大鼠肝纤维化的疗效优于各单一治疗组.
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