Gastric acid secretion is a dynamic and intricate process and in parietal cells H+-K+-ATPase is the last and critical element in this step. The principal stimulants of acid secretion are as follows, paracrine (histamine released by enterochromaffin cells), endocrine (gastrin released by G cells), and neurocrine (acetylcholine released by postganglionic enteric neurons). These agents interact with receptors in parietal cells to activate downstream protein kinases through cyclic adenosine monophosphate and calcium ion, translocating the acid-secreting pump (H+-K+-ATPase) of cytoplasmic tubulovesicle into apical membrane where it pumps H+ outside the cell in exchange of K+ at the expense of a molecule of ATP. This article reviewed the recent advances in study on gastric acid secretion related receptors and signal transduction pathway in parietal cells.%背景:胆汁反流是常见现象,其对胃上皮细胞生长的影响尚不明确.目的:探讨胆汁酸体外对胃癌细胞株MKN-28生长的影响.方法:采用MTT法测定不同浓度牛磺石胆酸钠(TLC)和甘氨鹅脱氧胆酸钠(GCDC)对胃癌细胞株MKN-28生长的影响.结果:第1～6 d,60～300 μmol/L TLC可明显抑制MKN-28细胞生长,且呈剂量依赖性.300 μmol/L GCDC干预24 h可显著抑制MKN-28细胞生长.结论:脂溶性胆汁酸TLC和水溶性胆汁酸GCDC体外均可直接抑制胃癌细胞生长.
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