目的：探讨天花粉蛋白（TCS）体外对前列腺癌细胞（PC3）生长的抑制作用及可能机制。方法采用噻唑蓝（MTT）检测TCS对PC3细胞的抑制作用。流式细胞术（ FCM）检测TCS对PC3细胞周期的影响，Western印迹检测TCS对ERK、p－ERK及细胞周期调节蛋白Cyclin D1表达的影响。结果 MTT 结果显示 TCS 能有效抑制 PC3细胞的生长，具有时间及剂量依赖性。流式细胞检测发现 TCS能够将 PC3细胞阻滞于 G1期， Western 印迹检测TCS能抑制ERK磷酸化及细胞周期调节蛋白Cyclin D1的表达。结论 TCS对PC3细胞的增殖具有明显的抑制作用，其作用机制可能与抑制丝裂原活化蛋白激酶（ MAPK）细胞增殖信号通路及降低Cyclin D1表达，从而诱导细胞发生G1期阻滞有关。%[Abstrca t] Obj ective To explore the inhibition effect of Trichosanthin (TCS) on prostate cancer PC3 cells and investigate the pos-sible mechanism .Methods MTT assay was used to detect the proliferation inhibition effect of TCS on PC 3 cells,cell cycle was analyzed by flow cytometry.Expressions of p-ERK and Cyclin D1 protein were detected by Western blot .Results TCS could inhibit the growth of PC 3 cell in a time and dose dependent manner .FCM detection showed that TCS could arrest PC 3 cells in G1 Phase.Western blot found that the protein expressions of p-ERK and Cyclin D1 were decreased after treated with TCS .Conclusions TCS could inhibit the proliferation of PC 3 cells by down-regulating the mitogen activated protein kinase pathway and inducing the G 1 phase arrest.
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