Objectives:To explore and analyze the value of weakly positive antibody in serum in window phase on the selecting the anti -HIV detection reagent.Methods:Three out of top eight domestic anti HIN ELISA reagent in 2010 report of Chinese disease control and prevention center were chosen.240 serum samples waiting for in-spection and quality control and 20 weakly positive antibodies in serum in window phase were randomly selected and detected.Detection quality indicators of three kinds of reagents were observed and compared.Results:Quality control serum S /CO value of reagent A and reagent C had significant difference (χ2 =5.28,P <0.05);Quality control ser-um S /CO value of reagent B and reagent C had significant difference (χ2 =5.65,P <0.05);Quality control serum S /CO value of reagent A and reagent B had no significant difference (χ2 =2.53,P >0.05).Sensitivity of reagent A, B and C was 100.00%,70.90% and 59.20% respectively,with significant difference between reagent A and reagent B (χ2 =4.53,P <0.05),and significant difference between reagent A and reagent C (χ2 =5.28,P <0.05),and significant difference between reagent A and reagent B (χ2 =2.66,P >0.05).Conclusion:In the study of anti -HIV reagent test,attention should be paid to selecting reagent with higher sensitivity.Weakly positive antibody in ser-um in window phase is of outstanding value in selecting anti -HIV detection reagent,which is worthy of promotion.%目的：探究与分析窗口期弱阳性抗体血清在选择抗－HIV 检测试剂中的重要意义。方法：于我国疾病预防控制中心在2010年报告中排名前8位中的3种国产抗 HIV ELISA 试剂，对随机选取的240份待检血清、质控血清及20份窗口期弱阳性抗体血清进行检测，观察与对比3种试剂的各项检测质量指标情况。结果：试剂 A 与试剂 C 质控血清 S ／CO 值相比具有显著差异（χ2＝5.28，P ＜0.05）；试剂 B 与试剂 C质控血清 S ／CO 值相比具有显著差异（χ2＝5.65，P ＜0.05）；试剂 A 与试剂 B 质控血清 S ／CO 值相比无明显差异（χ2＝2.53，P ＞0.05）。试剂 A 的敏感性为100.00％，试剂 B 的敏感性为70.90％，试剂 C 的敏感性为59.20％，试剂 A 与试剂 B 敏感性相比具有显著差异（χ2＝4.53，P ＜0.05）；试剂 A 与试剂 C 敏感性相比具有显著差异（χ2＝5.28，P ＜0.05）；试剂 B 与试剂 C 敏感性相比无明显差异（χ2＝2.66，P ＞0.05）。结论：在对抗 HIV 检测试剂的研究中需注意选择对两者均有较高敏感性的试剂，借助窗口期弱阳性抗体血清测定抗－HIV 检测试剂的临床应用价值突出，值得推广。
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