目的 了解淮北3所医院临床分离的36株铜绿假单胞菌氨基糖苷钝化酶(AME)基因存在情况.方法 采用MIC法测定临床分离菌株对3种氨基糖苷类及其他11种临床常用抗菌药物的敏感性;聚合酶链反应(PCR)法检测所测菌株中9种AME基因.结果 36株临床分离铜绿假单胞菌对阿米卡星、庆大霉素和妥布霉素的耐药率分别为62.2%、70.3%和81.1%;27株(75.0%)检出AME基因,ant(3″)-Ⅰ、aac(6')-Ⅱ、aac(6')-Ⅰ、aac(3)-Ⅱ和ant(2″)-Ⅰ检出率分别为63.9%、58.3%、50.0%、38.9%和36.1%,未检出aac(3)-Ⅰ、aac(3)-Ⅲ、aac(3)-Ⅳ、aph(3')-Ⅳ基因.结论 淮北3所医院临床分离的铜绿假单胞菌对氨基糖苷类抗生素耐药严重,AME携带率高且基因型的分布有明显的地区性.%Objective To investigate the genotypes of aminoglycoside-modifying enzymes (AMEs) produced by Pseudomonas aeruginosa isolated from Huaibei Miner's General Hospital.Methods Minimum inhibitory concentration of 36 strains of P. aeruginosa to 3 aminoglycoside antibiotics was determined. AME genes were amplified by polymerase chain reaction (PCR). Results The P. aeruginosa isolates were resistant to multiple antibiotics. Most (62.2% to 81.1%) were resistant to aminoglycosides. And 27 strain (75.0%) carried one or more types of AME genes, including ant(3″)-Ⅰ (63.9%), aac(6')-Ⅱ (58.3%), aac(6')-Ⅰ (50.0%), aac(3)-Ⅱ (38.9%) and ant(2″)-Ⅰ (36.1%). The aac(3)-Ⅰ, aac(3)-Ⅲ, aac(3)-Ⅳ, aph(3')-Ⅳ genes were not identified.Conclusions The study indicates that the P. aeruginosa isolates in Huaibei are multi-resistant to aminoglycoside antibiotics. The prevalence of AMEs-positive strains is high.
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