Objective To monitor Staphylococcus infection in a hospital,and trace the source of Staphylococcus isolated from infectious wound-related hospital environment by molecular analysis. Methods By combination of fluorescence quanti-tative polymerase chain reaction (FQ-PCR)and culture method,environmental specimens related to 5 patients with wound infection were taken and performed multilocus sequence and MecA gene typing analysis. Results A total of 71 environmen-tal specimens were taken,Staphylococcus accounted for 36.62% (n= 26),88.46% (23/26 )of which were MecA+ methi-cillin-resistant strains. 77.78% (7/9)of Staphylococcus aureus (S. aureus)and 95.00% (19/20)of coagulase negative Staphylococcus (CNS)were methicillin-resistant strains. Multilocus sequence analysis revealed that ST239 (n= 6)was the most common sequence type in S. aureus;Staphylococcal cassette chromosome mec (SCCmec)analysis showed that the ma-jor type of S. aureus was typeⅢ,and CNS were typeⅢandⅣ. Conclusion Staphylococcus is common in healthcare-associated infection,and most Staphylococcus are multidrug-resistant,continuous monitor on drug-resistant Staph-ylococcus is necessary,and risk of Staphylococcus variant to medical institutes need to be paid attention.%目的：对某医疗机构葡萄球菌感染情况进行持续监测，对创面感染患者相关医源性环境检出的葡萄球菌进行分子溯源分析。方法采用实时荧光聚合酶链反应（PCR）和分类培养鉴定相结合的方法，对该医疗机构5例创面感染患者相关的医源性环境进行采样检测，并进行金黄色葡萄球菌多位点序列分析和MecA基因的分型分析。结果采集医源性环境标本71份，葡萄球菌阳性26份（36．62％），其中23份检出甲氧西林耐药（MecA＋）株（88．46％，23/26）。金黄色葡萄球菌甲氧西林耐药株占77．78％（7/9），凝固酶阴性葡萄球菌甲氧西林耐药株占95．00％（19/20）。多位点序列分析结果显示，分离的金黄色葡萄球菌以ST239为主（6株）；SCCMec 分析，金黄色葡萄球菌以Ⅲ型为主，凝固酶阴性葡萄球菌以Ⅲ、Ⅳ型较多。结论医源性感染中葡萄球菌污染较多，且多为耐药株，应加强对耐药性葡萄球菌的持续监测，关注其变异对医疗机构医院感染的风险。
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