目的 建立武当地区马尾松松针中总黄酮及莽草酸含量测定方法.方法 采用紫外分光光度法,以芦丁为对照品,在波长500 nm处测定总黄酮的含量.采用HPLC测定莽草酸含量,色谱柱为Fortis Xi C8柱(5 μm,250 mm×4.6 mm),流动相为乙腈-0.4%磷酸溶液(8:92,V/V),流速为0.9 mL/min,检测波长为213 nm,柱温为30 ℃,进样量为20 μL.结果 芦丁在8.26~49.54 μg范围内与吸光度呈良好的线性关系(r=0.9994),平均加样回收率为99.2%,RSD=1.94%.莽草酸在10.26~61.56 μg范围内与峰面积呈良好的线性关系(r=0.9994),平均加样回收率为99.5%,RSD=1.93%.马尾松松针中总黄酮含量为28.33 mg/g,莽草酸含量为15.25 mg/g.结论 本方法简便、快速、准确、重复性好,可作为武当地区马尾松松针中总黄酮及莽草酸的含量测定方法.%Objective To establish a method to determine the contents of total flavonoids and shikimic acid in pine needles of Pinus massoniana Lamb.in Wudang Area.Methods Rutin was used as reference standard,and the content of total flavonoids in pine needles of Pinus massoniana Lamb. was determined by UV spectrometry at wavelength of 500 nm. The content of shikimic acid was determined by HPLC-DAD. The Fortis Xi C8 column (5 μm, 250 mm × 4.6 mm) was adopted with acetonitrile - 0.4% phosphoric acid solution (8:92, V/V) as mobile phase at the flow rate of 0.9 mL/min. The detection wavelength was 213 nm; the column temperature was 30 ℃; the injection volume was 20 μL. Results The linear range was 8.26-49.54 μg for rutin (r=0.999 4) with an average recovery of 99.2%, RSD=1.94%. The linear range was 10.26-61.56 μg for shikimic acid (r=0.999 4) with an average recovery of 99.5%,RSD=1.93%.The contents of total flavonoids in pine needles of Pinus massoniana Lamb.was 28.33 mg/g, and shikimic acid was 15.25 mg/g, respectively. Conclusion The method is simple, rapid, accurate and reproducible. It can be used for the content determination of total flavonoids and shikimic acid in pine needles of Pinus massoniana Lamb. in Wudang Area.
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