目的 探讨颐脑解郁复方对脑卒中后抑郁(PSD)大鼠行为学和海马CA1区病理损伤的干预作用.方法 将168只SPF级SD大鼠随机分为正常组、假手术组、卒中组、PSD组、西药组和中药组,正常组、假手术组各24只,其余组各30只.正常组不干预,假手术组不插入线栓,卒中组仅行大脑中动脉阻塞手术,PSD组、西药组、中药组卒中术后1周应用慢性束缚应激1周复合单笼饲养法制备PSD模型.造模开始时,西药组灌胃盐酸氟西汀,中药组灌胃颐脑解郁复方,其余各组灌胃蒸馏水,每日1次.第2、4、8周末,各组大鼠进行旷场试验等行为学测试,HE染色观察海马CA1区病理变化.结果 除第2周外,同一时间点中药组大鼠各项行为学评分均高于PSD组.同一时间点中药组大鼠海马CA1区较PSD组结构完整,细胞排列整齐,形态正常.结论 颐脑解郁复方可改善大鼠PSD症状,并对海马CA1区具有保护作用.%Objective To investigate the intervention effects ofYinao Jieyu Prescription on the behaviors and damages in hippocampal CA1 area of the rats with post-stroke depression (PSD).Methods Totally 168 SPF male SD rats were randomly divided into normal group, sham-operation group, stroke group, PSD group, Western medicine group and TCM group. There were 24 rats in the normal group and sham-operation group, and 30 rats in the other groups. Rats in the normal group received no intervention. Rats in the sham-operation group received no suture. Rats in the stroke group were given middle cerebral artery occlusion operation and normally fed after operation. Rats in the PSD group, Western medicinal group and TCM group were made into PSD models by chronic immobilization stress for one week and individual battery to the end. At the inception of modeling, Western medicine group received fluoxetine hydrochloride for gavage; TCM group receivedYinao Jieyu Prescription for gavage; other groups received distilled water for gavage, once a day. At the end of week 2, 4, and 8, the morphology of the hippocampal CA1 area in each rat was observed by microscope after HE stained.Results Except for the week 2, at the same time point, the behavior scores of the rats in the TCM group were higher than those in the PSD group. At the same time point, the CA1 region of the hippocampus in the TCM group was more complete than the PSD group, and the cells were arranged neatly and in normal morphology.ConclusionYinao JieyuPrescription can improve the symptoms of PSD rats, and has protective effects on hippocampal CA1 area.
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