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首页> 中文期刊> 《中国中医药信息杂志》 >百事乐胶囊对慢性应激抑郁大鼠海马磷脂酰肌醇-3激酶信号通路相关因子的影响

百事乐胶囊对慢性应激抑郁大鼠海马磷脂酰肌醇-3激酶信号通路相关因子的影响

         
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Objective To observe the effects of Baishile Capsules on the hippocampal PI3K signaling pathway in chronic mild unpredictable stress (CUMS) rats; To discuss its mechanism of action for anti-depression. Methods SD rats were randomly divided into 6 groups, control group, model group, fluoxetine group, Baishile Capsules high-, medium- and low-dose groups, with 10 rats in each group. The cums depression model was established, and gavage for medication was conducted at the same time for 21 days in a row. The behavioral changes of rats in each group were detected by the novel feeding experiment and Open-field experiment; monoamine neurotransmitter in serum were detected by ELISA; the expressions of PI3K, AKT and SGK1 in rat hippocampus were detected by immunofluorescence and Western blot test. Results Compared with the control group, feeding latency of rats in model group was significantly prolonged (P<0.01), horizontal and vertical activities were significantly reduced (P<0.01); the content of serum monoamine neurotransmitter decreased (P<0.01); the expressions of PI3K and AKT in hippocampus decreased, while the expression of SGK1 increased (P<0.01, P<0.05). Compared with the model group, Baishile Capsule high- and medium-dose groups can significantly shorten the incubation period of feeding rats (P<0.05), and increase the number of horizontal and vertical activities (P<0.01); the 5-HT and NE levels in serum were significantly elevated (P<0.05, P<0.01); the expressions of PI3K, AKT in hippocampal increased; the expression of SGK1 decreased (P<0.05, P<0.01). Conclusion Baishile Capsules can alleviate the depression like behavior in rat models, and regulate key factors of hippocampal PI3K signaling pathway, so as to exert antidepressant effects.%目的 观察百事乐胶囊对慢性轻度不可预见性应激(CUMS)大鼠海马磷脂酰肌醇-3激酶(PI3K)信号通路关键因子表达的影响,探讨其抗抑郁的作用机制.方法 将SD大鼠随机分为空白组、模型组、氟西汀组和百事乐胶囊高、中、低剂量组,每组10只.采用CUMS加孤养的方式建立抑郁模型,造模同时灌胃给药,连续21 d.采用新奇摄食实验和Open-field实验评价行为学变化,ELISA检测血清单胺神经递质,免疫荧光和Western blot检测大鼠海马PI3K、蛋白激酶B(AKT)、血清糖皮质激素调节蛋白激酶1(SGK1)表达.结果 与空白组比较,模型组大鼠摄食潜伏期显著延长,水平和垂直活动次数明显减少(P<0.01),血清单胺神经递质含量下降(P<0.01),海马PI3K、AKT表达显著降低且SGK1表达显著升高(P<0.05,P<0.01);与模型组比较,百事乐胶囊高、中剂量组大鼠摄食潜伏期显著缩短(P<0.05),水平和垂直活动次数显著增加(P<0.01),血清单胺神经递质含量显著升高(P<0.05,P<0.01),海马PI3K、AKT显著增加且SGK1表达显著降低(P<0.05,P<0.01).结论 百事乐胶囊可通过缓解模型大鼠抑郁样行为,调控海马PI3K信号通路关键因子,发挥其抗抑郁的功效.

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