Objective To study the uptake property of punicalagin in Caco-2 cells.Methods Caco-2 cell monolayer model was used to research the effects of punicalagin on cell uptake in different times, concentrations, pH values, temperatures and P-gp inhibitors. HPLC was used to determine the content of punicalagin in cells.Results The linearity of punicalagin concentration curve was found in the range of 0.024 8-0.992μg (r=0.997 9). Uptake determination of punicalagin in Caco-2 cells absorbed a certain amount of time, concentration and pH dependence and P-gp inhibitors could significantly increase the punicalagin uptake.Conclusion This method is simple and sensitive to determine the punicalagin concentration in Caco-2 cells, which is mainly passively transported. P-gp is involved in punicalagin transport process.%目的 研究安石榴苷在Caco-2细胞的摄取特性.方法 采用Caco-2细胞单层模型研究安石榴苷在不同时间、浓度、pH 值、温度、P-糖蛋白抑制剂条件下对细胞摄取的影响.采用高效液相色谱法测定安石榴苷在细胞中的含量.结果 安石榴苷在0.024 8~0.992μg范围内呈良好线性关系(r=0.997 9).安石榴苷在Caco-2细胞上的摄取呈现一定的时间、浓度和pH值依赖性,且P-糖蛋白抑制剂能显著增加安石榴苷的摄取量.结论 该方法操作简单、灵敏,安石榴苷在Caco-2细胞上的摄取主要是被动转运,P-糖蛋白参与安石榴苷的转运过程.
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