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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Assessing the Fate and Bioavailability of Glucosinolates in Kale (Brassica oleracea) Using Simulated Human Digestion and Caco-2 Cell Uptake Models
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Assessing the Fate and Bioavailability of Glucosinolates in Kale (Brassica oleracea) Using Simulated Human Digestion and Caco-2 Cell Uptake Models

机译：利用模拟人体消化和Caco-2细胞摄取模型评估甘蓝苷（Brassica Oleracea）中葡糖苷的命运和生物利用度

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摘要

Glucosinolates and their hydrolysis products were characterized in fresh and in in vitro gastric and intestinal digesta of Dinosaur kale (Brassica oleracea L var. palmifolia DC). In fresh kale, glucoraphanin, sinigrin, gluconapin, gluconasturtiin, glucoerucin, glucobrasscin, and 4-methoxylglucobrassicin were identified. After 120 min of gastric digestion, the levels of glucoraphanin, sinigrin, and gluconapin decreased, and no glucoerucin or glucobrasscin was detected. However, a concomitant increase in the glucosinolate hydrolysis products allyl nitrile, 3-butenyl isothiocyanate, phenylacetonitrile, and sulforaphane was observed. This trend continued through intestinal digestion. After 120 min, the levels of allyl nitrile, 3-butenyl isothiocyanate, phenylacetonitrile, and sulforaphane were 88.19 +/- 5.85, 222.15 +/- 30.26, 129.17 +/- 17.57, and 13.71 +/- 0.62 pmol/g fresh weight, respectively. Intestinal digesta were then applied to Caco-2 cell monolayers to assess the bioavailability. After 6 h of incubation, no glucosinolates were detected and the percentage of total cellular uptake of the glucosinolate hydrolysis products ranged from 29.35% (sulforaphane) to 46.60% (allyl nitrile).
机译：氨基糖苷和水解产物的特征在于新鲜和体外胃和肠道毒素的胃羽衣甘蓝（Brassica Oleracea L Var。Palmifolia DC）。在新鲜的羽衣甘蓝中，鉴定了葡萄花素，葡萄花，葡聚糖，葡聚糖，葡聚糖素，葡糖苷素，葡萄糖霉素和4-甲氧基葡聚糖蛋白。在胃消化120分钟后，葡萄花素，SINIGROM和葡聚糖蛋白的水平降低，并且没有检测到葡糖酸或葡糖霉菌。然而，观察到葡萄糖苷水解产物烯丙基腈，3-丁烯基异硫氰酸酯，苯乙腈和亚磺烷的伴随增加。这种趋势通过肠道消化持续。 120分钟后，烯丙基腈，3-丁烯基异硫氰酸盐，苯基乙腈和亚磺酸盐的水平为88.19 +/- 5.85,222.15 +/- 30.6,129.17 +/-17.57和13.71 +/- 0.62 pmol / g鲜重，分别。然后将肠道减少施用于Caco-2细胞单层以评估生物利用度。孵育6小时后，检测到葡萄糖素酸盐，葡萄糖酸盐水解产物的总细胞吸收百分比范围为29.35％（氟化物）至46.60％（烯丙基丁腈）。

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