Objective To identify the common Tibetan herb Dong-Na Duan-Chi by molecular pharmacognosy. Methods Ethnopharmacology investigation was carried out and local herbalists were visited to observe which plants were being used as Dong-Na Duan-Chi. Then, nuclear ribosomal DNA ITS sequences were amplified and sequenced for molecular identification. Results The botanic origins of Dong-Na Duan-Chi in Tibet Autonomous Region were authenticated as two species, i.e. Veronica eriogyne H.Winkl. and Veronica ciliata Fisch.. The sequences of ITS for Veronica eriogyne and Veronica ciliata are 551 bp and 552 bp in size. The results showed that nrDNA ITS sequences could be used to discriminate V.eriogyne and V.ciliata from closely related species. Conclusion nrDNA ITS sequences can be used as the molecular markers to identify the Tibetan herb Dong-Na Duan-Chi from other species of the same genus Veronica L.%目的：对常用藏药冬那端赤进行分子鉴定。方法在民族植物学考察、经典分类学鉴定基础上，对植物核糖体DNA内转录间隔区(rDNA ITS)序列进行测定与分析。结果确定西藏产冬那端赤的2种基源植物为玄参科婆婆纳属(Veronica L.)狭果组(Sect.Stenocarpon)的毛果婆婆纳Veronica eriogyne H.Winkl.、长果婆婆纳V.ciliata Fisch.，其nrDNA ITS序列分别为551、552 bp。序列分析显示，以ITS序列为分子标记，可区别两者及其近缘种。结论 nrDNA ITS 序列在藏药冬那端赤及其近缘种中具有很好的种间分辨率，可用于冬那端赤的分子生药学鉴定。
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