Objective To study the immunologic mechanism of electroacupuncture at Sanyin points on chronic abacterial prostatitis rats. Methods Forty-eight male rats were divided randomly into normal group, model group, drug group and electroacupuncture group, 12 rats in each group. The animal model was made by SC purified prostate protein twice with immunologic adjuvant, and the rats in electroacupuncture group and drug group were treated by electroacupuncture at Sanyin points and feeding Cernilton for 15 days. The changes of prostate weight, index, pathology and contents of IgG, expressions of IL-2 and IL-8 in prostate homogenate were observed. Results Compared with normal group, the prostate weight and index were significantly increased in model group (P<0.05), the content of IgG was significantly increased (P<0.05), cytokine IL-2 and IL-8 were highly expressed (P<0.05, P<0.01), prostate tissue’s morphology and structure were significantly impaired in model group. Compared with model group, the prostate weight and index were significantly decreased in electroacupuncture group after treatment (P<0.05 or P<0.01), pathological changes reduced significantly, the content of IgG decreased significantly (P<0.05), the expressions of cytokine IL-2 and IL-8 were significantly decreased (P<0.05, P<0.01), and the pathological manifestation of chronic inflammation was significantly improved. Conclusion Electroacupuncture at Sanyin points can reduce the secretion of local prostate antibody IgG, regulate the expression of cytokine IL-2 and IL-8, protect the morphology and structure of prostate tissue from damage. Electroacupuncture at Sanyin points can adjust local immunologic function and promote the recovery of prostate.% 目的观察电针“三阴穴”对慢性非细菌性前列腺炎大鼠模型免疫功能的影响,并探讨其作用机制。方法将48只雄性SD大鼠随机分为空白组、模型组、药物组、电针组。皮内注射同种异体雄性SD大鼠前列腺蛋白提纯液,辅以双重免疫佐剂复制自身免疫性慢性非细菌性前列腺炎大鼠模型。造模后电针组、药物组分别给予电针“三阴穴”、舍尼通灌胃治疗15 d。观察各组动物前列腺组织湿重及指数、形态结构,用放免法检测前列腺组织中抗体IgG含量,局部组织细胞因子白细胞介素(IL)-2、IL-8的变化。结果造模后模型组前列腺组织湿重及指数与空白组比较显著增高(P＜0.05),病理观察显示,模型组动物前列腺组织形态及结构明显受损；与空白组比较,模型组大鼠前列腺组织匀浆中IgG含量明显增高(P＜0.05),局部细胞因子IL-2、IL-8高表达(P＜0.05,P＜0.01)。治疗后,电针组前列腺湿重及指数均明显下降(P＜0.05,P＜0.01),病理变化明显减轻；局部前列腺组织IgG含量明显降低(P＜0.05),局部细胞因子IL-2、IL-8表达显著下降(P＜0.05,P＜0.01)。结论电针“三阴穴”能够减少非细菌性前列腺炎大鼠局部抗体IgG的分泌,调节局部细胞因子IL-2、IL-8的表达,减轻组织局部免疫亢进,抑制前列腺组织形态结构的损伤,从而起到治疗慢性非细菌性前列腺炎的作用。
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