目的研究少林正骨精的质量控制方法。方法采用薄层色谱法对制剂中的独活、当归进行定性鉴别。采用高效液相色谱法测定处方中血竭的有效成分血竭素的含量，以乙腈-0.05 mol/L磷酸二氢钠溶液(50∶50)为流动相，检测波长为440 nm。结果定性鉴别的特征斑点清晰，专属性强，阴性对照无干扰；血竭素在3.997～43.97 ng范围内线性良好，加样回收率为101.26%(RSD＝2.84%)。结论定性、定量方法准确可靠、重复性好，能有效控制少林正骨精的内在质量。%Objective To study the quality control method of Shaolin Zhenggujing tincture. Methods Angelicae Pubescentis Radix and Angelicae Sinensis Radix in the preparation were identified by TLC. The content of dracorhodin, the effective component of Resina Draconis, was determined by HPLC with acetonitrile-0.05 mol/L sodium dihydrogen phosphate (50∶50) as a mobile phase and 440 nm as the detection wavelength. Results Characteristic spots of TLC were clear with strong specificity and the negative test showed no interference. There was a good linear relationship for dracorhodin in the range of 3.997-43.97 ng and the recovery was 101.26% (RSD=2.84%). Concludion The qualitative and quantitative methods are accurate, reliable and repeatable, it can effectively control the inner quality of Shaolin Zhenggujing tincture.
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