Objective To establish the method to determine the contents of rhein, emodin and rnchrysophanol in Zhitonggao. Methods RP-HPLC method was adopted with Hypersil ODS C18 as column.rnThe mobile phase consisted of methanol-water (85∶15) with a flow rate of 1.0 mL/min. The detection rnwavelength was 254 nm. Results The linear correlation of rhein was good in the range of 0.208－1.040 μg rn(r ＝0.999 0, n＝5) and the recovery was 98.37% (RSD＝0.57%, n＝5). The linear correlation of emodin was rngood in the range of 0.192－0.96 μg (r ＝0.999 7, n＝5) and the recovery was 98.45% (RSD＝0.99%, n＝5).rnThe linear correlation of chrysophanol was good in the range of 0.596－2.980 μg (r ＝0.999 5, n＝5) and rnthe recovery was 98.18% (RSD＝1.50%, n＝5). Conclusion The method is simple, sensitive and accurate,rnwith good reproducibility. It can be applied to control the quality of the preparation.%目的 建立测定止痛膏中大黄酸、大黄素和大黄酚含量的方法.方法 采用反相高效液相色谱法,十八烷基键合硅胶柱分离大黄酸、大黄素和大黄酚,以甲醇-水(85∶15)为流动相,流速为1.0 mL/min,检测波长254 nm.结果 大黄酸在0.208～1.040 μg范围内线性关系良好(r＝0.999 0,n＝5),平均加样回收率为98.37%(RSD＝0.57%,n＝5);大黄素在0.192～0.96 μg范围内线性关系良好(r＝0.999 7,n＝5),平均加样回收率为98.45%(RSD＝0.99%,n＝5);大黄酚在0.596～2.980 μg范围内线性关系良好(r＝0.999 5,n＝5),平均加样回收率为98.18%(RSD＝1.50%,n＝5).结论 本方法 简单、灵敏、准确、重复性好,可用于该制剂的质量控制.
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