Objective To analyse the latent inqredients making the warps result in the clinical examination of the human beings multiple cytokins in sera.Methods examinating the clinical samples both short and long period observed groups using quantified ELISA on the status of the strict exsamination conditions and operation, and making statistical analysis for the exmaination data.Results short period observed groups consisit of 40 patients with symptomatic gallstone for elective cholesystectomy wich include 20 casese of open cholesystectomy (OC) and 20 cases of laparoscopic cholesystectomy (LC).The value of the IL-6 show transient rise, and there was significant defference compared with that before operation. (P<0.01,0.01 and 0.05 respective ); but the standard deviation and the value of the S/X- show the trendency to increasing relied on time. the long period observed group contains 28 cases of patient with chronic hepatitis C virus infection that treatment by IFN-a injection. The averages of IL-2,IL-4 and IL-12 detection values When the end of the treatment(24th weeeks of treatment) and following up investigationg (48th weeeks of begaining tretment), were increased by shawed as -0.7% & 31.0%, -47.5% $ -24.8%,and 50.5% & -0.5%.For the standard deviation and the S/X- coefficient rate was too large(between 61.8% and 168.6%), there were no significant difference (P were all >0.05) compared with that before treatment.Conclusion It could make the warps examination result of the human beings multiple cytokins in sera because of the patient's different responseve to stimulate of physics,chemistry and biology during the observation.%目的 通过两组实验数据平行对比探讨细胞因子临床检测数据偏差的潜在原因.方法 在严格实验条件与操作的前提下,采用定量ELISA对短程与长程观察组临床标本进行检测,并对实验数据进行分析.结果 短期观察组开腹手术与腔镜手术者IL-6测值均呈短暂一过性升高,与术前比较均有显著性差异(各时点比较P分别<0.01,0.01及0.05);但其标准差及变异系数CV(S/X-)值有依时性升高的趋势;对长期观察组IFN-a治疗结束及随访期间的IL-2、IL-4与IL-12三者检测均值与治疗前均值进行比较,其升降幅度分别为-0.7%与31.0%, -47.5%与-24.8%,以及50.5%与-0.5%.但因标准差(S)以及变异系数过大(61.8%-168.6%之间),各时点测值与治疗前之间均未见显著性差别(P均>0.05),结论不同患者对环境中理化与生物学因子刺激的反应各异,由此造成的测值波动将干扰对细胞因子科研与临床的评价.
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