目的：构建烟曲霉 kipA 基因突变株。方法通过 In-Fusion 技术将 kipA 基因的上下游基因与抗性基因顺序链接，以潮霉素抗性基因替换靶基因，达到用同源重组手段敲除烟曲霉 kipA 基因的目的，以 realtime-PCR 对基因突变株进行鉴定和验证。结果 RT-PCR 结果显示，成功构建烟曲霉 kipA 基因突变株。结论烟曲霉 kipA 基因突变株的构建，为后续烟曲霉极性生长与血管侵袭性的研究提供了实验基础。%Objective To construct the kipA mutant strain.Methods The vector containing upper and downstream genes of kipA linked with hygromycin resistance gene in the middle was constrctied via In-Fusion assembly.The kipA gene of As-pergillus fumigatu was replaced with hygromycin resistance gene via the method of homologous recombination.The mutant strain was identified and validated by realtime-PCR.Results The kipA deletion strains were successfully constructed.Conclu-sions Successful construction of kipA mutant strain would provide the foundation for the research of polarized growth and vascular invasion of Aspergillus fumigatus .
展开▼
