Objective:To analyze the concentration of urine angiotensinogen (AGT) in patients with chronic kidney disease (CKD),and to explore the significance of urinary AGT excretion in the diagnosis of CKD.Methodology:Clinical data of CKD and non CKD patients from the CKD resource repository in a cross-sectional survey were collected.CKD patients were divided into 3 groups according to estimated glomerular filtration rate (eGFR):CKD 1 group,CKD 2 group and CKD 3 group.Urine AGT was detection by ELISA method.Results:A total of 95 CKD patients and 413 non CKD controls were enrolled in this study.Urine AGT in CKD group was significantly higher than that in non CKD group (243.9±47.9 vs 158.4±25.14,P＜0.01),With the decrease of eGFR,urine AGT level increased,it was 213.6±34.5 ng/L,251.4±17.2 ng/L and 314.9±27.6 ng/L in CKD 1 group,CKD 2 group and CKD 3 group respectively (F=81.103,P＜0.01).Multiple linear regression analysis showed that urine AGT was negatively correlated with eGFR (P＜0.01) and positively correlated with urine albumin/creatinine (P＜0.01) and serum creatinine (P＜0.01).Conclusion:Urinary AGT excretion was significantly elevated in CKD patients,and it was negatively correlated with eGFR.Urine AGT may be a useful biomarker of CKD.%目的:探讨尿液血管紧张素原(angiotensinogen,AGT)与慢性肾脏病(CKD)的关联性,以及尿AGT对CKD的诊断价值. 方法:从新疆维吾尔自治区墨玉县农村维吾尔族成人CKD流行病学调查的资源标本库中抽取CKD和非CKD样本,按估算的肾小球滤过率(eGFR)将CKD患者分为CKD 1期组、CKD 2期组、CKD 3期组.尿AGT用ELISA方法检测.one-way-ANOVA分析组间差异,多因素分析采用多重线性回归分析. 结果:95例CKD患者和413例非CKD对照者进入本研究.CKD组尿液AGT明显高于非CKD组(243.97±47.86vs158.43±25.14,t=16.892,P＜0.01);随着eGFR下降,尿液中AGT呈升高趋势,CKD 1期组、CKD 2期组和CKD 3期组患者尿液AGT分别为213.63±34.47 ng/L,251.39±17.17 ng/L和314.97±27.58 ng/L,各组间有显著差异(F=81.103,P＜0.01).多重线性回归分析表明,尿液AGT与eGFR呈负相关(P＜0.01),与尿白蛋白/肌酐(P＜0.01)和血清肌酐(P＜0.01)呈正相关. 结论:CKD患者尿AGT高于非CKD患者,与eGFR下降呈负相关.提示尿AGT可作为CKD诊断和病程进展的生物学标志物.
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