To evaluate the correlation between the whole blood cyclosporine A (CsA) concentrations by HPLC and TDx. Methodology: A total of ninety two samples from renal allograft recipients were determined by HPLC and TDx assay. Regression analyses were performed ( HPLC results as dependant. TDx as independant variable) for the evaluation of correlation. Results: TDx results were higher (25.4%) than those from HPLC. There existed good correlation with least-squares equation Y = 0. 826X + 8. 318 ( n = 92, r = 0. 967). Conclusion: There were significant differences in determination result of CsA between HPLC system and TDx.%目的:研究高效液相色谱(HPLC)法和荧光偏振免疫(TDx)法测定人全血环孢素A(CsA)浓度的相关性. 方法:分别用HPLC法和TDx法测定92例肾移植受者的CsA血药浓度,测定值用t检验和线性回归方法进行分析. 结果:TDX法和HPLC法测定全血CsA浓度具有相关性,线性回归方程为:Y=0.826X +8.318(r=0.967,n=92,P＜0.01),TDx测定值比HPLC测定值平均高25.44％. 结论:TDx测定值明显高于HPLC测定值,两种测定方法所得结果存在显著性差异.
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