随着转化医学理念和研究思路的引入,以组学为基础的系统生物学解决复杂问题的优越性得以突显,即通过宏观思路,组装系统方案,解决微观问题,同时通过不同组学在不同研究层面的相互补充,加深了对生命真谛的认识.例如,早期医学研究主要集中在基因水平,由此提出基因组学研究思路,但随着人类基因组计划和全基因组测序完成,科学家们发现很多情况下染色体序列不同,甚至转录水平的变化并不导致表型改变,因而将目光投向基因翻译后的产物蛋白质,并提出蛋白质组学研究方法.磷酸化蛋白质组学是该领域的一个新分支,通过单次试验可以静态评估上千个蛋白的磷酸化情况,发现蛋白新的生理功能,更重要的是可以全局性地研究蛋白磷酸化的动力学变化,观察病理状态下不同蛋白间的相互作用及信号传导的变化,并为寻找药物靶点提供了全新的解决途径.本文将就磷酸化蛋白质组学的特点及其在生命科学中的应用前景作一简述.%With the development of translational medicine, we realize that systems biology solve complex biological problems (i. e. macro issues,sophisticated programs,and solution the problem) . At the same time,we have a more understanding in the real meaning of life. For example, the early medical researches focus on the gene, and developed genomics. But in the end of the Human Genome Project,the scientists found the transcriptional level changes which do not change phenotypic. Phosphoproteomics is a branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a post-translational modification. Phosphorylation is a key reversible modification that regulates protein function, subcellular localization, complex formation, degradation of proteins and therefore cell signalling networks. Quantitative methods can assess the relative change in phosphorylation for several thousand sites in a single experiment, detect novel phosphorylation sites, study dynamic phosphorylation events, understand cell signaling pathways and discovery the new drug target . Here, the phosphoproteomics apply in the life sciences, introduce the characteristics and defect of phosphoproteomics are reviewed.
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