Objective To explore the protective effect of calycosin on cerebral ischemia-reperfusion injury in rats and its mechanism.Methods Focal rat brain ischemic model was established by middle cerebral artery occlusion (MCAO).SD rats were randomly divided into MCAO group, vehicle group and calycosin group.Neurological dysfunction score (NDS) and the infarct volume were measured at 48 h after ischemia-reperfusion.Brain tissues were collected pre-operation, 6 h, 24 h and 48 h post-operation for the measurement of contents of superoxide dismutase (SOD) , catalase (CAT) , glutathione peroxidase (GSH-PX) and maleic dialdehyde ( MDA ).Results The NDS outcome in the calycosin group was better than that of the MCAO group and vehicle group (P ＜0.05) ;the brain infarct volume of the calycosin group was significantly smaller than that of MCAO group and vehicle group (P ＜ 0.05) ;the activity of SOD, CAT, GSH-PX were increased significantly but the content of MDA was decreased in ischemic brain tissues in the calycosin group, compared with those in MCAO group (P ＜0.05).Conclusion Calycosin has neuroprotecfive effect on the focal cerebral ischemic injury, which may be related to the increase of the antiperoxidase activity and the decrease of oxygen free radicals.%目的 探讨毛蕊异黄酮对大鼠脑缺血再灌注损伤的保护作用及机制.方法 采用大脑中动脉阻塞(MCAO)制备局灶性脑缺血模型,将SD大鼠随机分为MCAO组、溶剂对照组(vehicle组)以及毛蕊异黄酮处理组(calycosin组),缺血再灌注48 h后观察各组神经功能学评分和脑梗死容积大小.术前、术后6 h、24 h、48 h取脑组织样本,分别检测其超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)的变化.结果 calycosin组神经功能评分优于MCAO组和vehicle组(P<0.05);calycosin组脑梗死容积小于MCAO组和vehicle组(P<0.05);与MCAO组相比,calycosin组的脑组织SOD、CAT、GSH-PX活力升高,MDA 的含量下降,差异均具有显著性(P<0.05).结论 毛蕊异黄酮对大鼠局灶性脑缺血再灌注损伤有一定的神经保护作用,其保护作用与毛蕊异黄酮能降低脑内氧自由基水平、控制脂质过氧化程度有关.
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