目的 探讨表皮生长因子样结构7基因( EGFL7)、第10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)在脑胶质瘤中的表达和相互关系.方法 应用免疫组化方法检测56例脑胶质瘤和8例脑外伤内减压脑组织中EGFL7和PTEN的表达.结果 EGFL7蛋白在脑胶质瘤组织和正常脑组织中的阳性表达率差异有显著统计学意义(P＜0.01)；高度恶性胶质瘤组阳性表达率明显高于低度恶性胶质瘤组(P＜0.01).PTEN蛋白在脑胶质瘤组织和正常脑组织中的阳性表达率差异有显著统计学意义(P＜0.01)；随着胶质瘤恶性程度的增加,PTEN蛋白的表达相应降低(P＜0.01).EGFL7与PTEN在各级胶质瘤组织的表达呈负相关(r=-0.382,P＜0.01).结论 EGFL7基因可能直接参与了胶质瘤的发生、侵袭过程；PTEN基因突变或丢失、灭活导致PTEN蛋白表达的缺失或减弱在胶质瘤的发生、侵袭发展过程中起重要作用；EGFL7表达与PTEN表达具有明显的负相关性,EGFL7高表达与PTEN表达下降在胶质瘤的浸润发展过程起协同作用.%Objective To study the expression of epidermal growth factor-like domain 7 (EGFL7) and phosphatase and tensin homology deleted on chromosome ten (PTEN) in human glioma tissues and their correlations. Methods The expressions of EGFL7 and PTEN in 56 cases of human glioma and 8 cases of normal brain tissues were detected by immunohistochemistry and their clinicopathologic features were analyzed. Results There was a significant difference in the positive rates of EGFL7 between normal brain tissues and glioma (P<0.01). The positive reaction rate of EGFL7 was increased with the increase of tumor malignancy (P<0.01). There was a significant difference in the positive rates of PTEN between normal brain tissues and glioma (P < 0.01). The expression of PTEN was decreased with the increase of tumor malignancy ( P < 0. 01). There was a negative correlation between EGFL7 and PTEN in glioma (r = -0.382, P < 0.01). Conclusion EGFL7 may be a potential indicator for evaluating tumorigenesis and invasion; absence of PTEN expression might play an important role in tumorigenesis and invasion; there is a negative correlation between EGFL7 and PTEN; high expression of EGFL7 and decreased expression of PTEN promotes the invasion of gliomas.
展开▼
