Objective To study the skill of fetal umbilical blood sampling guided by ultrasound and summa-rize the experience of sampling, and to improve the success rate and reduce complications.Methods One hundred and seventy-six clinically suspected abnormal pregnant women were performed the umbilical blood sampling guided by ultrasound.All the data were statistically analyzed.Results The success rate of the first attempt was 79.5%(140/176), and the final success rate was 94.3%(166/176).Conclusion Real-time ultrasound guidance in umbilical blood sampling has the advantage of positioning accuracy, which plays a decisive role in improving the success rate of first attempt and reducing complications of umbilical blood sampling.Umbilical blood sampling is an effective remedy of prenatal diagnosis during the middle-late pregnancy.%目的：探讨超声引导下行产前诊断脐静脉穿刺技巧，总结穿刺经验，提高成功率，降低并发症发生率。方法收集176例超声引导下行脐静脉穿刺患者的临床资料并进行统计分析。结果一次进针穿刺成功率为79．5％（140／176），最终穿刺成功率为94．3％（166／176）。结论采用超声实时引导，定位准确对提高脐静脉穿刺一次性成功率，减少并发症起决定性作用，是妊娠中晚期产前诊断的有效补救措施。
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