Objective To observe the effect of parathyroid (rhPTH1-34) combined with simvastatin (SIM) on the differentiation of osteoblasts and the mRNA expression of OPG and RANKL in vitro.Methods The neonatal rat osteoblasts were cultured in vitro.The osteoblasts were treated with 10 -9 mol/L rhPTH1-34 combined with different concentrations of SIM (10 -8 mol/L, 10 -7 mol/L, and 10 -6 mol/L, respectively) .The activity of ALP was assessed using PNPP colorimetric assay.The mRNA expression of OPG and RANKL was detected using semi-quantitive RT-PCR.Results Single use of rhPTH1-34 or SIM promoted ALP activity, increased the expression of OPG, and reduced the expression of RANKL (P<0.05).Compared with that in single SIM treatment group, the ALP activity in rhPTH1-34 combined with SIM treatment group increased more significantly, and the expression of OPG was also promoted, while the expression of RANKL was down-regulated ( P<0.05) .Conclusion rhPTH1-34 combined with SIM has additive effect on the differentiation and metabolism of osteoblasts.%目的：联合甲状旁腺激素（ rhPTH1－34）和辛伐他汀（ SIM）在体外对乳鼠颅盖骨成骨细胞分化及骨保护素（ OPG）和核因子κB受体活化因子配体（ RANKL）基因表达的影响。方法以乳鼠成骨细胞为体外试验模型，rhPTH1－34（10－9 mol／L）联合不同浓度SIM（10－8、10－7、10－6mol／L）作用于体外培养的乳鼠成骨细胞，采用对硝基苯磷酸盐（PNPP）法测定碱性磷酸酶（ALP）活性；RT－PCR法测定OPG和RANKL基因的表达。结果 rhPTH1－34和SIM单独给药均可促进成骨细胞ALP活性及OPG基因、降低RANKL基因表达（ P＜0．05）；两者联合后与SIM单独作用组比较， ALP活性明显增加，并能协同促进 OPG、降低RANKL基因表达（P＜0．05）。结论 rhPTH1－34和SIM联合应用对成骨细胞分化和代谢有协同作用。
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