Objective To exlpore the expressions and clinical significances of hypoxia inducible factor-l alpha (HIF-1c),estrogen receptor alpha (ERα) and matrix metalloproteinases-9 (MMP-9) in papillary thyroid carcinoma (PTC).Methods The expressions of above-mentioned factors in specimens of PTC and para-carcinoma thyroid tissues from 88 PTC patients were detected by immunohistochemical method (IHC).And the relationships among clinical parameters and their expressions were analyzed.Results The positive rates of HIF-1 α,ERα and MMP-9 proteins in PTC were 70.45％,62.5％ and 84.1％ respectively,which were higher than those of the para-carcinoma tissues (all P ＜ 0.01).And their positive rates in the specimens with lymph node metastasis or at clinical stage Ⅲ ～ Ⅳ were higher than those without lymph node metastasis or at clinical stage Ⅰ ～ Ⅱ (P ＜ 0.05 or P ＜ 0.01),which indicated that their positive rates were related to the lymph node metastasis and clinical stage of PTC.The positive rate of ERαin specimens with diameter more than 2 cm was higher than that in those with diameter equal to or less than 2 cm (P ＜ 0.05).Their positive rates were positively correlated with each other (all P ＜ 0.01).Conclusion Enhanced expressions of HIF-1 α and ERαmay participate in the clinical course of PTC via the high expression of MMP-9.%目的 探讨乏氧因子-1 α(hypoxia inducible factor-1α,HIF-1α)、雌激素受体理(estrogen receptor α,ERα)与基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)在甲状腺乳头状癌中的表达及与临床因素的关系.方法 采用免疫组化技术对88例乳头状癌及癌旁组织中HIF-1α、ERα和MMP-9的表达情况进行检测,分析三者与临床参数的关系.结果 甲状腺乳头状癌(papillary thyroid carcinoma,PTC)组织中HIF-1 α、ERα和MMP-9三者阳性率(70.45％,62.5％和84.1％)均高于癌旁组织(P ＜0.01);PTC组织中HIF-1 α、ERα和MMP-9阳性率与淋巴结转移和临床分期有关.在淋巴结转移组和临床Ⅲ～Ⅳ期病例3者阳性率均高于无淋巴结转移组和临床Ⅰ～Ⅱ期病例(P ＜0.05或P＜0.01);ERα阳性率在直径＞2 cm的病例高于直径≤2 cm者(P ＜0.05);HIF-1α与ERα、HIF-1α与MMP-9阳性率呈正相关(均为P＜0.01);ERα阳性率与MMP-9也呈正相关(P＜0.01).结论 甲状腺乳头状癌中HIF-1α、ERα高表达可能通过MMP-9参与PTC的临床进程.
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