目的：探讨微小RNA（ miRNA）-21对低氧缺血损伤PC12细胞的影响。方法：体外培养PC12细胞，建立氧糖剥夺（ OGD ）损伤模型。细胞随机分为对照组、OGD组、阴性对照序列＋OGD组、miRNA-21 inhibitor ＋OGD组和miRNA-21 mimic＋OGD组。通过采用CCK-8、real-time PCR、Western blot 等技术探讨miRNA-21对OGD损伤PC12细胞的影响和机制。结果：降低miRNA-21的表达，受OGD损伤的PC12细胞活力明显下降；增加miR-NA-21的表达，受OGD损伤的PC12细胞活力明显增加。进一步发现miRNA-21促进OGD损伤PC12细胞的AKT磷酸化。结论：miRNA-21明显增加OGD损伤PC12细胞的活力，其机制可能与激活PI3K／AKT信号通路有关。%AIM:To investigate the effect of microRNA ( miRNA)-21 on the PC12 cells with hypoxic-ischemic damage.METHODS:The PC12 cells were cultured in vitro, and the cell model of oxygen-glucose deprivation (OGD) was established .In accordance with the following requirements , the cells were randomly divided into control group , OGD group, negative control sequence +OGD group, miRNA-21 inhibitor +OGD group and miRNA-21 mimic+OGD group. The effects and mechanism of miRNA-21 on the protection of PC12 cells from OGD damage were determined by CCK-8 as-say, real-time PCR and Western blot .RESULTS:Decrease in the expression of miRNA-21 by transfection with miRNA-21 inhibitor inhibited the viavility of the PC 12 cells subjected to OGD damage .Increase in the expression of miRNA-21 by transfection with miRNA-21 mimic promoted the viability of the PC 12 cells subjected to OGD damage .It was further con-firmed that miRNA-21 promoted the AKT phosphorylation in OGD-damaged PC12 cells.CONCLUSION: miRNA-21 sig-nificantly increases the viability of PC 12 cells subjected to OGD damage , which may be related to the activation of PI 3K/AKT signaling pathway .
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