目的：探讨microRNA-1284（ miR-1284）与胃癌的相关性及分子机制。方法：Real-time PCR检测63例胃癌患者的胃癌组织和匹配周围正常胃组织中miR-1284的表达水平及分析其与临床病理特征之间的关系。通过慢病毒载体的构建及包装生成上调miR-1284慢病毒载体并转染胃癌SGC-7901细胞，上调miR-1284后采用real-time PCR验证细胞miR-1284表达水平，CCK-8法检测细胞活性，流式细胞术检测细胞周期和凋亡，划痕实验检测细胞迁移能力，生物信息学软件预测miR-1284潜在的靶基因，real-time PCR测定靶基因JAG1表达水平，Western blot-ting检测JAG1、Notch1和NF-κB的表达水平。结果：66.67％胃癌患者胃癌组织miR-1284表达明显低于正常胃组织（P＜0.05），miR-1284的表达在不同年龄、性别、TNM分期和淋巴结转移情况患者间的差异无统计学意义（P＞0.05），但在不同组织学分级患者间的差异有统计学意义（P＜0.05）。与LV-NC-GFP组和control组比较，LV-miR-1284组的miR-1284表达水平和细胞凋亡显著升高，细胞周期阻滞于G0／G1期，细胞活性和细胞迁移能力减弱， JAG1、Notch1和NF-κB的表达水平降低，均有显著差异（ P＜0.05）。结论：miR-1284可能通过调控其靶基因JAG1而发挥抑制胃癌发生发展的作用。%AIM:To evaluate the correlation between microRNA-1284 (miR-1284) and gastric cancer, and to investigate the underlying mechanism.METHODS: The expression of miR-1284 was examined by real-time PCR in 63 gastric cancer ( GC) tissue samples and 63 non-malignant adjacent tissue samples.The correlation between miR-1284 and the clinicopathological feature of GC was analyzed.Lentiviral vector containing miR-1284 was constructed and transfected into GC SGC-7901 cells.After transfection, the expression of miR-1284 was examined by real-time PCR.The cell activity was evaluated by CCK-8 assay.The cell cycle and apoptosis were determined by flow cytometry.The ability of cell migra-tion was measured by wound-healing assay.The potential target gene of miR-1284 was predicted by online bioinformatic softwares.The expression of JAG1 mRNA was examined by real-time PCR.The protein levels of JAG1, Notch1 and NF-κB were analyzed by Western blotting.RESULTS:Compared with non-malignant adjacent tissue samples, the results of real-time PCR showed significant downregulation of miR-1284 in 42 GC tissue samples ( P<0.05 ) .The expression level of miR-1284 was not significantly associated with age and gender of the patients, tumor size, TNM staging and lymph node metastases (P>0.05), but significantly associated with histologic grading (P<0.05).Compared with LV-NC-GFP group and control group, after transfection of miR-1284 in LV-miR-1284 group, the expression of miR-1284 was significantly in-creased (P<0.05), the percentages of apoptotic cells and the cells in G0/G1 phase were significantly increased (P<0.05), the cells activity and ability of migration were significantly decreased (P<0.05), and the expression of JAG1, Notch1 and NF-κB was significantly decreased (P<0.05).CONCLUSION:The inhibitory effect of miR-1284 on gastric cancer may be associated with the regulation of its targeting gene JAG1.
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