[ ABSTRACT] AIM:To investigate the effect of edaravone ( ED) on the expression of caspase-3 and caspase-12 in the juvenile rat hippocampus after status convulsion ( SC) .METHODS: Juvenile male Sprague-Dawley rats were ran-domly divided into normal saline ( NS) control group, SC group and ED treatment group.The rats in each group were fur-ther divided into 5 subgroups according to different time points.The rats in SC group were kindled into epilepsy by lithium-pilocarpine chemical method.The protein expression of caspase-3 and caspase-12 was determined by immunohistochemistry methods.The mRNA expression of caspase-3 and caspase-12 was detected by RT-PCR.RESULTS:(1) The IA value of caspase-3 positive cells in 24~72 h SC group increased compared with NS group.With ED intervention, the IA value of caspase-3 positive cells decreased as compared with 48~72 h SC group.The results of RT-PCR showed that the mRNA ex-pression of caspase-3 was similar to the changes of protein.( 2 ) The results of immunohistochemistry showed that the IA value of caspase-12 positive cells in 12~72 h SC group increased compared with NS group.With ED intervention, the IA value of caspase-12 positive cells decreased as compared with 24~72 h SC group.The results of RT-PCR showed that the mRNA expression of caspase-12 was similar to the changes of protein.( 3 ) In ED group, Ⅴ grade convulsion was lower than that in SC group, and the latent period of seizures in ED group was significantly longer than that in SC group.CON-CLUSION:Edaravone inhibits the expression of caspase-3 and caspase-12 in pilocarpine-induced seizures in rat hippo-campus, suggesting that edaravone has protective effect against the damage caused by status convulsion.%目的：探讨依达拉奉（ edaravone，ED）对幼年大鼠惊厥持续状态（ status convulsion，SC）后海马中caspase-3和caspase-12表达的影响。方法：将195只SD幼年雄性大鼠随机分为生理盐水对照组（ NS组）、SC组和ED组；各组又均按时点分为4 h、12 h、24 h、48 h和72 h 5个亚组，每组13只。采用氯化锂-匹鲁卡品化学点燃法制备幼年大鼠SC模型。应用免疫组织化学法检测大鼠海马中caspase-3和caspase-12蛋白表达情况，逆转录多聚酶链反应（ RT-PCR）法检测caspase-3和caspase-12 mRNA的表达。结果：（1）免疫组织化学法检测SC 24～72 h组幼年大鼠海马中caspase-3表达增强，与NS组比较差异有统计学意义（ P＜0.05）；与SC组比较，ED 48～72 h组的caspase-3表达明显降低，差异有统计学意义（P＜0.05）；RT-PCR法检测结果显示caspase-3 mRNA的表达趋势与蛋白基本相似。（2）免疫组织化学法检测SC组幼年大鼠海马中12～72 h组caspase-12表达增强，与NS组比较差异有统计学意义（P＜0.01）；与SC组比较，ED 24～72组caspase-12表达明显降低，差异有统计学意义（P＜0.05）；RT-PCR法检测结果显示caspase-12 mRNA的表达趋势与蛋白基本相似。（3）经比较，ED组Ⅴ级惊厥率较SC组低，差异有统计学意义（ P＜0.05）；且ED组惊厥潜伏期也较SC组明显延长，差异有统计学意义（ P＜0.05）。结论：依达拉奉可抑制匹鲁卡品所致SC大鼠海马caspase-12和caspase-3的表达，提示依达拉奉对SC引起的脑损伤可能有保护作用。
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