AIM:To explore the effect of GATA6 gene silencing on apoptosis of hepatocellular carcinoma Huh-7 cells.METHODS:RNA interference vectors of the target gene GATA6 mediated by lentivirus were constructed in vitro to transfect the hepatocellular carcinoma cell line Huh-7.The apoptotic rate of transfected cells was measured by flow cytome-try.The protein expression of GATA6, NF-κB and Bcl-2 in transfected cells was determined by Western blotting.RE-SULTS:The transfection efficiency was 57.4%.The mRNA and protein expression of GATA6 reduced significantly after the carcinoma cell line Huh-7 being transfected by RNA interference vectors mediated by lentivirus.The apoptotic rate of the carcinoma cells with silent GATA6 gene was significantly increased (P<0.05).The protein expression levels of NF-κB and Bcl-2 were also significantly decreased.CONCLUSION: Lentiviral vector-mediated RNA interference of GATA6 has an inhibitory effect on the expression of the gene itself, and promotes the apoptosis of hepatocellular carcinoma cells.Regu-lation of the apoptosis-related protein expression by the NF-κB signaling to influence the proliferation and apoptosis of hepa-tocellular carcinoma cells might be one of the possible mechanisms.%目的：研究GATA6基因沉默对肝癌Huh-7细胞凋亡的影响，同时探讨可能的作用机制。方法：构建靶向GATA6基因的慢病毒干扰载体，用流式细胞术确定转染效率，用RT-PCR和Western blotting法检测其对人类肝细胞癌细胞株Huh-7的干扰效果，流式细胞术检测细胞凋亡比例， Western blotting法检测各转染组Huh-7细胞NF-κB及Bcl-2蛋白表达。结果：靶向GATA6基因的慢病毒干扰载体感染肝癌Huh-7细胞后，转染效率为57.4％。GATA6在mRNA和蛋白水平的表达明显下降。 GATA6沉默的肝癌Huh-7细胞凋亡比例增加（ P＜0.05），NF-κB和Bcl-2蛋白水平表达降低。结论：利用慢病毒载体干扰技术沉默GATA6基因的表达可以明显促进Huh-7细胞凋亡，其机制可能通过NF-κB信号通路调节凋亡相关蛋白的表达，进而影响细胞的凋亡。靶向GATA6的RNA干扰技术在肝癌的基因治疗中具有一定的研究价值。
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