目的：探讨葡萄球菌肠毒素A（staphylococcal enterotoxin A ， SEA）对甲磺酸伊马替尼（imatinib me-sylate，IM）抑制T淋巴细胞活化的影响。方法：2 mg/L SEA 和50 nmol/L IM联合作用于Jurkat细胞24 h后，用实时荧光定量PCR技术检测CD3ε和ζ链mRNA表达水平变化；Western blotting 技术检测CD3ε和ζ链蛋白水平变化。结果：单纯IM组CD3ε和ζ链mRNA及蛋白表达均表现下调；SEA与IM联合用药组可以逆转IM下调CD3ε和ζ链mRNA及蛋白表达水平作用。 SEA拮抗IM抑制作用中，对CD3ε链mRNA表达上调的作用明显大于CD3ζ链。结论：SEA能拮抗IM对T细胞CD3ε和ζ链表达的抑制作用。%AIM:To investigate the effects of staphylococcal enterotoxin A (SEA) on the inhibition of T lym-phocyte activation induced by imatinib mesylate (IM).METHODS:Jurkat cells were stimulated with SEA (2 mg/L) and IM (5 nmol/L) for 24 h.The mRNA expression of CD3εandζchains was measured by real-time fluorescence quantitative PCR.The protein levels of CD3ε and ζchains were detected by Western blotting .RESULTS: The expression of CD3εand ζchains at mRNA and protein levels was down-regulated in the Jurkat cells stimulated by IM alone .These down-regu-lations of CD3εandζchains were reversed by the stimulation of IM combined with SEA .The antagonistic effect of SEA on IM-mediated inhibition of CD3εmRNA expression was significantly greater than that on CD 3ζmRNA.CONCLUSION:SEA antagonizes imatinib-mediated inhibitory effect on T cell activation .
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