• 主题
高级搜索  >
历史搜索 清空历史
首页> 中文期刊> 《中国病理生理杂志》 >HGF基因转染对裸鼠人淋巴瘤移植瘤的促进作用

HGF基因转染对裸鼠人淋巴瘤移植瘤的促进作用

         
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To explore the promotion effect of hepatocyte growth factor (HGF) gene transfection on human lymphoma xenografts in nude mice. METHODS: The model of human lymphoma xenograft in nude mice was established by transplantation of Raji cells, which were transfected with recombinant plasmid pVYTRO2-HGF harboring the HGF gene. The body weight of the nude mice and the tumor size were dynamically monitored and the tumor tissues were obtained after 8 weeks. Additionally, the methods of terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) and immunohistochemistry were used to detect the apoptotic index (AI) and microvessel density (MVD). RESULTS: The success rate of the human lymphoma xenografts in nude mice was 96.7%. The tumor volume in HGF transfection group was significantly greater than that in HGF transfection + VP-16 group and control groups ( non-transfection group and empty vector group). The tumor volume in HGF transfection + VP-16 group was also bigger than that in control groups. No difference of the tumor volume between non-transfection group and empty vector group was observed. AI in HGF transfection group was substantially lower than that in control groups. AI in HGF transfection + VP-16 group showed a little higher than that in HGF transfection group, yet was still lower than that in control groups. MVD in HGF transfection group was extraordinary higher than that in control groups, but decreased after VP-16 induction (P <0. 01) , which was still higher than that in control groups. CONCLUSION: HGF gene transfection significantly promotes the growth of human lymphoma xenografts in nude mice and substantially inhibits the apoptosis presumably owing to promoting tumor angiogenesis and inhibiting tumor cell apoptosis.%目的:探讨肝细胞生长因子(HGF)基因转染对裸鼠人淋巴瘤移植瘤的促进作用及其机制.方法:采用HGF基因重组质粒pVITRO2-HGF转染的Raji细胞建立裸鼠皮下人淋巴瘤移植瘤模型,动态监测裸鼠体重和肿瘤大小；8周后获取瘤组织,分别采用脱氧核糖核酸末端转移酶介导的缺口末端标记技术(TUNEL)和免疫组化检测移植瘤组织的细胞凋亡和微血管密度(MVD),并进行相关分析.结果:造模成功率为96.7％.HGF转染组的瘤体积明显大于HGF转染+VP-16组(P＜0.01)、未转染组与空载体组(P＜0.01),HGF转染+VP-16组也大于对照组(P＜0.01),未转染组与空载体组间无显著差异(P＞0.05).pVITRO2-HGF转染组凋亡指数显著低于对照组(P＜0.01),经VP-16诱导后凋亡增加(P＜0.01),但仍低于对照组(P＜0.01).pVITRO2-HGF转染组的MVD显著高于对照组(P＜0.01),但经VP-16诱导后血管增生降低(P＜0.01),但仍高于对照组(P＜0.05),对照组间无显著差异(P＞0.05).结论:HGF基因转染可显著促进裸鼠人淋巴瘤移植瘤的生长,明显抑制凋亡的发生,这一效应可能与其促进肿瘤血管新生和抑制肿瘤细胞凋亡有关.

著录项

  • 来源
    《中国病理生理杂志》 |2013年第1期|81-85|共5页
  • 作者

    赵行; 郑筱娇; 高洲; 沈蓉蓉; 岑东; 裴仁治; 罗建平; 吕建新;

  • 作者单位

    温州医学院浙江省医学遗传学重点实验室,浙江温州325027;

    温州医学院附属乐清医院,乐清市人民医院,浙江乐清325600;

    温州医学院浙江省医学遗传学重点实验室,浙江温州325027;

    温州医学院浙江省医学遗传学重点实验室,浙江温州325027;

    温州医学院浙江省医学遗传学重点实验室,浙江温州325027;

    温州医学院浙江省医学遗传学重点实验室,浙江温州325027;

    宁波市鄞州区疾病预防控制中心,浙江宁波315100;

    宁波市鄞州区疾病预防控制中心,浙江宁波315100;

    宁波市鄞州人民医院,浙江宁波315040;

    温州医学院浙江省医学遗传学重点实验室,浙江温州325027;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 病理生理学;
  • 关键词

    肝细胞生长因子; 基因转染; 淋巴瘤; 裸鼠; 异种移植物;

相似文献

  • 中文文献
  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号