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首页> 中文期刊> 《中国病理生理杂志》 >MALT1基因2种转录本在正常人和B细胞白血病患者中的表达特点

MALT1基因2种转录本在正常人和B细胞白血病患者中的表达特点

         
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AIM:To investigate the difference of the distribution and expression of mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1) transcripts in healthy individuals and B-cell leukemia patients.METHODS:Two pairs of primers were designed according to the sequences of different MALT1 transcripts.RT-PCR was performed to detect the distribution of MALT1 gene in peripheral blood mononuclear cells (PBMC) from 8 healthy individuals,8 patients with B-cell acute lymphocytic leukemia (B-ALL) and 8 patients with B-cell chronic lymphocytic leukemia (BCLL).The mRNA expression of MALT1 in all samples from the 3 groups was determined by real-time PCR.RESULTS:Expected amplicons were amplified by the 2 primer pairs,and 2 PCR products of different sizes were amplified by the first primer pair (the larger one included exons 5,6,7,8 and 9,and the small one included exons 5,6,8 and 9).Moreover,the MALT1 transcript variant 1 was conformed by the second primer pair.All the PCR products were confirmed by the nucleotide sequencing analysis.Two transcripts of MALT1 were identified in all samples from both healthy individuals and Bcell leukemia patients,and the expression level of MALT1 was predominant in transcript variant 2.The gray level of MALT1 transcript variant 1 in B-ALL patients was significantly higher than that in healthy individuals,whereas the MALT1 transcript variant 2 was significantly lower than that in healthy group.However,no significant difference of the gray level was found in the 2 MALT1 transcript variants in B-CLL group as compared with healthy group.In addition,the expression level of MALT1 mRNA in B-ALL group (median =1.253) was lower than that in healthy group (median =1.976,P =0.05),while no significant difference of the expression level of MALT1 mRNA between B-CLL patients and healthy individuals was found.CONCLUSION:Two transcripts of MALT1 have common distribution in both healthy individuals and B-cell leukemia patients,while the significant differences of distribution of MALT1 transcript variants and the expression level of MALT1 mRNA are characterized in B-ALL patients.The abnormal expression of MALT1 gene might involve in the genesis and development of leukemia via MALT1 signaling pathway.%目的:比较正常人和B细胞白血病患者外周血中黏膜相关淋巴样组织淋巴瘤转位基因1(MALT1)2种异构体的分布和表达差异.方法:根据MALT1基因的结构特点,设计2对引物,通过建立2种转录本的逆转录聚合酶链式反应(RT-PCR)方法分析8例B细胞急性淋巴细胞白血病(B-ALL)、8例B细胞慢性淋巴细胞白血病(B-CLL)和8例正常人的外周血单个核细胞(PBMC)中MALT1的表达情况.实时定量PCR分析各组样本的MALT1 mRNA表达水平.结果:所有样本中均能得到所预期的PCR产物,其中第1对引物可以扩增出2条片段,分别为MALT1转录本1(包含第5、6、7、8、9外显子)和MALT1转录本2(包含第5、6、8、9外显子),第2对引物只能扩增出1条包含转录本1的片段,所有PCR产物均通过核苷酸序列分析证实.MALT12种转录本均在外周血中有表达,并且MALT1转录本2表达水平高于转录本1.通过灰度分析发现,在B-ALL中,MALT1转录本1的灰度与正常人相比显著增高(P＜0.05),而MALT1转录本2显著降低(P＜0.05)；在B-CLL中,MALT12种转录本的灰度与正常人相比无显著差异(P＞0.05).此外,B-ALL中,MALT1 mRNA表达水平(中位数:1.253)低于正常人(中位数:1.976)(P=0.05)；而B-CLL中,MALT1 mRNA的表达水平与正常人相比无显著差异(P＞0.05).结论:MALT12种转录本均表达于正常和B细胞白血病样本中,但B-ALL中两者的表达水平有所差异,同时,B-ALL的MALT1 mRNA表达水平也有所降低.B-ALL中MALT1基因表达的异常可能影响MALT1信号通路而与疾病的发生发展相关.

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