AIM:To evaluate the effects of bone marrow - derived mesenchymal stem cells ( MSCs ) on en-graftment of hematopoietic stem/progenitor cells in sensitized mice. METHODS:Mouse bone marrow - derived MSCs were cultured by adherent culture method. MSCs combined with or without hematopoietic stem/progenitor cells were implanted into the sensitized mouse model, which was established by allogeneic splenocyte transfusion, and were divided into 6 groups; MSC intervention groups, including sensitized mice with MSCs on day 11 , sensitized mice with MSCs on day 0 and sensitized - mice with MSCs both on day 11 and day 0; control groups, including sensitized mice without MSC intervention, non - sensitized mice without MSC intervention and non - sensitized mice without MSCs or transplantation of hematopoietic stem/progenitor cells. The survivors were assessed after transplantation and hematopoietic recovery was monitored weekly including hematological change, immune function reconstruction, bone marrow cell recovery, chimera analysis and graft - versus - host disease development. RESULTS: Compared with different control groups, MSC intervention did not prolong the survival rates of the sensitized model mice after lethal irradiation. CONCLUSION: Under the experimental conditions, MSC combined with C57BL/6 bone marrow hematopoietic stem/progenitor cells fail to promote the growth of en-graftment in C57BL/6 allogeneic splenocyte -sensitized BALB/c mice in vivo.%目的:前期的研究已经证实致敏小鼠造血干/祖细胞移植植入失败率高.本研究拟通过骨髓间充质干细胞(MSCs)进行干预,观察能否提高造血干、祖细胞移植的植入率.方法:应用贴壁培养法体外培养正常小鼠骨髓MSCs,并分为6个实验组,包括实验组1:d11 MSCs干预的致敏组;实验组2:d0 MSCs干预的致敏组;实验组3:d11和d0 2次MSCs干预的致敏组;实验组4:无MSCs干预的致敏小鼠对照组;实验组5:无MSCs干预的正常小鼠(非致敏小鼠)移植对照组;实验组6:无MSCs干预的正常小鼠不移植对照组.观察指标包括生存分析、移植效果分析(血象改变、骨髓细胞恢复及嵌合分析等)和移植物抗宿主病(GVHD)检测,最终评估MSCs干预对各实验组异基因造血干/祖细胞移植植入率的影响效果.结果:与对照组(实验组4、5、6)比较,MSCs干预(实验组1、2、3)在2次异基因脾细胞注射法致敏的动物模型进行异基因造血干/祖细胞移植时,未能促进骨髓造血干/祖细胞移植的植入,也未能延长致敏动物移植后的生存时间.结论:体内应用1×106 MSCs干预,未能促进2次异基因1×106 C57BL/6小鼠脾细胞输注法建立的重度致敏模型异基因造血干/祖细胞移植的植入.
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