AIM: To identify the enhancement of relatively specific immune responses by thymopeptide and 4 polysaccharides isolated from traditional Chinese medicine in the mice bearing cervical cancer. METHODS: The model of cervical cancer - bearing mice was established by implantation of cervical cancer U14 cells into the armpit of the right fore-limb of the mice. Beginning from the 2nd day of tumor implantation, 4 polysaccharides [ including Lycium barbarum poly-saccharide ( LBP) , Angelica sinensis polysaccharide ( ASP) , Ganodema lucidum polysaccharide ( GLP) and Panax ginseng polysaccharide (PGP) ] , thymopeptide or saline solution were intragastric administered for 21 days according to the experi-mental design. The animals were then killed, the tumor inhibitory rate and spleen index in treatment groups were compared with those in control group. The splenic T -lymphocytes (TLC) and natural killer (NK) cells, and the content of IFN -γ and IL - 10 in the tumor tissues were also determined by flow cytometry and immunohistochemical methods. RESULTS: The order of tumor inhibitory rates was: ASP < PGP < thymopeptide < LBP < GLP. The effects of GLP, LBP and thy-mopeptide were statistically different from that of control group. No difference of tumor inhibition between PGP/ASP groups and control group was observed. The order of spleen index was: ASP < tumor control group < thymopeptide < GLP < PGP < LBP, that of CD4/CD8 enhancement was: PGP < ASP < GLP < thymopeptide < LBP, and that of CD49b + NK hyperplasia was: ASP < thymopeptide < LBP < GLP < PGP, with statistically significant differences among the groups. The changes of IFN -γ and IL - 10 in the tumor tissues showed that Thl/Th2 in thymopeptide group, LBP grouprnand GLP group shifted to Thl. No shift to Thl in PGP group and ASP group was observed. CONCLUSION: LBP, GLP and thymopeptide show significant inhibitory effects on tumor growth in U14 cervical tumor - bearing mice and can enhance the immune functions in the animals.%目的:比较4种中药多糖及胸腺肽对U14宫颈癌荷瘤鼠肿瘤生长、脾增生、脾内T淋巴细胞(TLC)、自然杀伤细胞(NK)及瘤组织内Th1/Th2的影响,试图找到对宫颈癌有相对特异性的免疫增强剂.方法: 建立U14宫颈癌荷瘤鼠模型,用生理盐水、中药多糖或胸腺肽连续21 d灌胃,处死后检测治疗组及对照组的抑瘤率和脾脏指数,采用流式细胞术和免疫组化法分析脾内TLC和NK细胞亚群以及瘤组织内IFN-γ和IL-10含量.结果:肿瘤抑制率排序:当归多糖(ASP)<人参多糖(PGP)<胸腺肽<枸杞多糖(LBP)<灵芝多糖(GLP),GLP、LBP和胸腺肽组与肿瘤对照组相比差异有统计学意义;PGP和ASP组与肿瘤对照组相比无显著差异.脾脏指数:ASP<肿瘤对照组<胸腺肽组展开▼
