目的:研究系统性红斑狼疮(SLE)患者外周血T细胞中Rho激酶(ROCK)活化情况,并探讨其对T细胞黏附和迁移的影响.方法:收集SLE患者33例,正常健康人12例和类风湿关节炎患者9例作为对照组.外周血T细胞分离采用RosettSep T细胞提取试剂盒提取,ROCK活性用磷酸化肌球蛋白磷酸酶靶亚基1(MYPT1)蛋白表达来表示,磷酸化MYPT1蛋白检测采用免疫印迹法,T细胞迁移采用Transwell小室测定.结果:新鲜分离的SLE患者外周血T细胞ROCK活性均显著高于正常对照组和类风湿关节炎组(均P＜0.05).SLE患者T细胞体外黏附和迁移能力显著高于正常对照组;ROCK特异性抑制剂Y27632显著抑制SLE患者T细胞黏附和迁移.结论:SLE患者存在T细胞ROCK活化异常;ROCK可能参与调控SLE T细胞的黏附和迁移;抑制T细胞异常的ROCK活性可能有助于SLE的治疗.%ATM; To investigate the role of Rho kinase (ROCK) in the regulation of adhesion and migration of the T cells from systemic lupus erythematosus (SLE) patients. METHODS; The T cells were isolated by RosettSep T cell purification kit. ROCK activity was assessed by Western blotting. T cell migration was examined by Transwell chambers . RESULTS : Compared with the T cells from healthy controls and rheumatoid arthritis patients , the activity of ROCK in ex vivo T cells from SLE patients was significantly increased . In vitro, the adhesion and migration of the T cells from SLE pa -tients were also increased. Furthermore, the adhesion and migration of the T cells from SLE patients were inhibited by a specific ROCK inhibitor Y27632. CONCLUSION; The results indicate that ex vivo T cells from SLE patients exhibit increased activity of ROCK. Alteration of ROCK activity may contribute to the abnormal adhesion and migration of T cells from SLE patients.
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