目的:研究心肌成纤维细胞的运动特性,以及房颤与非房颤患者血清对成纤维细胞趋化运动诱导能力的差异.方法:用胰酶和胶原酶消化、差速贴壁法培养新生SD大鼠的心肌成纤维细胞,取3-4代采用Transwell装置检测房颤组血清与非房颤组血清诱导成纤维细胞运动的能力差异.结果:在各组血清的趋化诱导作用下,与对照组相比,迁移至下室的细胞数明显增多.其中持续性房颤组最多,非房颤房性心律失常组明显高于阵发性房颤组,对照组细胞数最少,各组比较均具有显著差异.Logistic回归分析表明,迁移至滤膜下的细胞数与左房直径以及房颤有相关性.结论:(1)各组血清的趋化诱导能力高于对照组,不同组间的差异说明心房纤维化是一个慢性、隐匿、迁延的过程.以修复为目的的成纤维细胞迁移浸润数量间接反映了心肌损伤的存在、范围和程度.(2)迁移浸润的细胞数变化先于左房直径变化,提示房颤和左房直径增大的正相关关系可能并非直接的因果关系,心房肌损伤后的纤维化可能参与其中.%AIM: To investigate the effects of the serum from the patients with atrial fibrillation ( AF) on the chemotaxis of rat cardiac fihroblasts.METHODS : Cardiac fibroblasts were isolated from the ventricles of neonatal Sprague - Dawley rats and primarily cultured with digestion and differential adhesion.The cells in 3 to 4 passages were used for Transwell chamber assay to determine the chemotatic effects of the sera.RESULTS: Compared with control group, the cells that migrated through the polycarbonate membrane were obviously increased in AF group.The strongest chemotaxis was induced by the serum from the patients with persistent atrial fibrillation( pers - AF) .The number of migrated cells in non - AF atrial arrhythmia( AA) group was higher than that in paroxysmal atrial fihrillation( paro - AF) group, and that in control group was the lowest.The results of multiple Logistic regression analysis showed that the migrated cells were related to AF and left atrial diameter.CONCLUSION : The chemotactic effect of AF serum is ohviously higher than that of control serum.The differences of AF sera among groups show that myocardial fibrosis is a chronic, insidious and delayed process.The migration and infiltration of cardiac fibroblasts indirectly reflect the presence, severity and extent of the myocardial damage.The changes of migrated cells precede the changes of left atrial diameter, indicating that the cause of fihrosis is more important, and the positive correlation between AF and left atrial diameter may not he the direct causality.
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