目的 了解强心苷类药物地高辛对非霍奇金淋巴瘤raji细胞侵袭能力的影响,并对其作用机制进行初步探讨.方法 选取河北医科大学第四医院病理科2010年10月至2013年12月手术切除的非霍奇金淋巴瘤组织标本30例,采用免疫组化S-P方法检测非霍奇金淋巴瘤T淋巴瘤侵袭转移诱导因子1(Tiam-1)及Ras相关的C3肉毒素底物1(Rac-1)蛋白表达情况;通过细胞侵袭实验以及实时荧光定量聚合酶链式反应(PCR)技术检测不同浓度地高辛作用于raji细胞后Tiam-1、Rac-1表达水平变化.结果 免疫组化结果显示,非霍奇金淋巴瘤中Tiam-1蛋白染色阳性率(70％)及Rac-1蛋白染色阳性率(73.33％)均高于正常淋巴结组织,差异有统计学意义(P＜0.05).相关分析结果显示,Tiam-1及Rac-1具有相关性,相关系数r=0.757 (P＜ 0.01).细胞侵袭实验结果显示,不同浓度组地高辛作用raji细胞48 h后,随着作用浓度的增加侵袭率下降,差异有统计学意义(P＜0.01).实时荧光定量PCR结果显示,不同浓度的地高辛分别作用于raji细胞48h,各组之间Tiam-1及Rac-1的相对表达量差异有统计学意义(P＜0.05),并随着作用浓度的增加相对表达量降低.结论 强心苷类药物地高辛可通过调节Tiam-1及Rac-1因子的表达对淋巴瘤侵袭及转移起到一定抑制作用.%Objective To study the effect of digoxin on the invasion ability of non-hodgkin lymphoma (NHL) raji cells,and to preliminarily investigate its mechanism of action.Methods The 30 tissue samplec of non-Hodgkin lymphoma were taken in the Pathological Department of the Fourth Hospital of Hebei Medical University from Oct.2010 to Dec.2013.(1) The protein expression of Tiam-1 and Rac-1 in N HL were detected by i mmunohistochemical S-P method.(2)With different concentration of digoxin,the changes in expression levels of Tiam-1 and Rac-1 within raji cells were detected via cell invasion test and qRT-PCR.Results (1)Immunohistochemical result indicated:the positive rates of protein Tiam-1 (70％) and Rac-1 (73.33％) in NHL were higher than those in normal lymph node tissues with statistical significance (P ＜ 0.05).Correlation analysis indicated there was correlation between Tiam-1 and Rac-1,with a correlation coefficient of r =0.757 (P ＜ 0.01).(2)Cell invasion test indicated:after 48 h treatment by digoxin of different concentrations,the invasion rate reduced with the increasing digoxin concentration,and the results were statistically significant (P ＜ 0.01).(3) qRT-PCR result indicated:after 48 h treatment by digoxin of different concentrations,statistical difference was observed in the relative expression levels of Tiam-1 and Rac-1 between groups;the relative expression levels reduced with the increasing digoxin concentrations (P ＜ 0.05).Conclusion Digoxin,a cardiac glycoside,is able to inhibit lymphoma invasion or metastasis to some extent via regulating the expressions of Tiam-1 and Rac-1.
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