The aim of this research is to study the transcription levels of the mismatch repair related genes of MMR (MSH2,MSH3,MLH1 and PMS1),BRCA1,p53 genes mRNA in DF-1 cells when infected with the avian leukosis virus subgroup J (ALV-J).DF-1 cells were infected with the ALV-J NX0101 strain and the p27 antigen was detected to confirm the infection.The total RNA was extracted to detect the expression of the mismatch repair related genes by real-time PCR.The transcription levels of MSH2,MSH3,MLH1,PMS1,and p53 mRNA were increased in DF-1 cells after ALV-J infection,but the levels of BRCA1 mRNA were decreased.These results demonstrated that MSH2,MSH3,MLH1,PMS1,BRCA1 and p53 genes response to the recognition and repair of DNA damage.This study provides a new idea for studying the mechanism of tumorigenesis induced by ALV-J.%为研究禽白血病病毒J亚群(ALV-J)感染DF-1细胞后错配修复相关基因MMR、BRCA1和p53mRNA的转录水平变化,本研究将ALV-J NX0101株接种DF-1细胞,感染7d后检测ALV-p27抗原,确定病毒感染是否成功.然后提取细胞中总RNA,采用荧光定量PCR以[3-actin基因作为内参对MMR (MSH2、MSH3、MLH1和PMS1)、BRCA1和p53基因的mRNA转录情况进行检测.结果表明,与正常对照细胞比较,病毒感染细胞中的MMR (MSH2、MSH3、MLH1和PMS1)和p53基因的mRNA转录水平升高,而BRCA1基因的mRNA转录水平降低.表明在ALV感染宿主细胞后MMR (MSH2、MSH3、MLH1和PMS)、BRCA1和p53参与了宿主细胞DNA损伤的识别及修复过程,这一结果为ALV的致瘤机理的研究提供了新的思路.
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