本研究于2013年3月在广东某猪场采集疑似伪狂犬病(PR)的猪脑组织.对脑组织进行病毒分离,通过透射电镜观察及PCR扩增gB和gE基因,并进行序列测定.结果表明,透射电镜显示该病毒粒子具有典型的疱疹病毒结构,PCR可以扩增出伪狂犬病病毒(PRV) gB和gE基因,经序列分析和生物学特性鉴定,证实该分离病毒株为我国近几年流行的PRV变异株,并将其命名为PRV-GD2013.小鼠致病性试验表现出典型的PR症状.通过以上实验可以确定该猪场感染病原为PRV.%This study was collecting the suspected pseudorabies-infected pigs' brain tissue in a pig farm in Guangdong province in March,2013.The virus was isolated from the brain tissue and then identified by transmission electron microscopy (TEM),and the gB and gE sequences were amplified by PCR.The results of TEM showed that the virus particles had a typical herpes virus structure,and the gB and gE genes of pseudorabies virus (PRV) was able to be amplified by PCR.The sequence analysis and biological characteristics comfirmed that the isolate was a mutant PRV,named as PRV-GD2013,compared with the PRVs isolated in China in recent years.In pathogenicity experiments,the mice infected with PRV-GD2013 showed typical pseudorabies symptoms.It could be confirmed that the pigs in the farm were infected with the PRV.
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