The study was based on the EST data of cDNA library of adult of Toxocaia canis. The Yolk protein gene of T. Canis (TcF-YP) was amplified by RT-PCR and cloned into pGEM-T vector. The sequence analysis shown that the of TcF-YP gene was 492 bp in length encoding 163 amino acid residues which shared 78% homology with YP of Ascaris suum. The gene expression patterns of TcF-YP between female and male adult of T. Canis revealed that TcF-YP was specifically expressed in female adult of T. Canis. Tissue expression patterns of TcF-YP in 6 tissues of female adult of T. Canis indicted that TcF-YP were highly expressed in uterus and intestine.%为克隆犬弓首蛔虫(T.canis)的卵黄原蛋白(YP)基因,本研究根据T.canis cDNA文库中的EST数据,采用RT-PCR方法扩增T.canis的YP基因(TcF-YP),并克隆至pGEM-T载体中进行测序.测序结果表明,TcF-YP基因片段大小为492 bp,编码163个氨基酸残基.同源分析显示,TcF-YP基因与猪蛔虫的卵黄原蛋白基因同源性最高,为78％.同时对该基因在T.canis雌虫、雄虫和雌虫各组织的表达谱进行研究,结果显示TcF-YP基因只在T.canis雌虫中高量表达,是雌虫特异的基因.对雌虫各组织的基因表达谱分析显示TcF-YP基因在子宫和肠中高量表达,在卵巢中有极微量的表达.本实验为TcF-YP基因的功能研究奠定了基础.
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