背景：动物实验和临床研究表明在大脑发育的早期进行功能剥夺可能会引起成年期神经和认知功能障碍，但是到目前为止关于嗅觉功能活动是否影响成年豚鼠梨状皮质的神经发生尚无报道。 目的：建立嗅觉剥夺模型观察嗅觉经验对成年豚鼠两侧梨状皮质的微管聚合蛋白、小白蛋白、谷氨酸脱羧酶67以及微管相关蛋白与神经元特异性核蛋白共表达的影响。 方法：将20只成年豚鼠随机平分成2组，建立嗅觉剥夺模型饲养3周和6周之后灌注取材，应用免疫组织化学技术观察各个时间点豚鼠自身两侧梨状皮质微管相关蛋白、小白蛋白和谷氨酸脱羧酶67的分布，免疫荧光观察微管相关蛋白与神经元特异性核蛋白的共表达。 结果与结论：3周和6周组嗅觉未剥夺侧梨状皮质表达的微管相关蛋白、小白蛋白和谷氨酸脱羧酶67阳性细胞数目均明显多于嗅觉剥夺侧梨状皮质，两侧相比差异有显著性意义(P<0.05)；3周组和6周组两侧梨状皮质微管相关蛋白与神经元特异性核蛋白的共表达细胞差异无显著性意义(P>0.05)。提示嗅觉经验对成年豚鼠梨状皮质神经元的成熟及分化起着重要促进作用。%BACKGROUND:Animal experiments and clinical studies have shown that, functional deprivation at the early development stage may possibly cause nerve and cognition dysfunction at adulthood. However, no research focuses on the effect of olfactory function on the neurogenesis in piriform cortex of adult guinea pigs. OBJECTIVE:To establish olfactory deprivation models and explore the effect of olfactory experience on the distribution of doublecortin, parvalbumin, glutamic acid decarboxylase 67 and the co-localization of doublecortin with neuron-specific nuclear-binding protein in piriform cortex of adult guinea pigs. METHODS:Twenty guinea pigs were randomly divided into two groups. Olfactory deprivation models were established through unilateral naris-occlusion and the occluded animals were al owed to survive 3 and 6 weeks, then the specimens were harvested. The distribution of doublecortin, parvalbumin, glutamic acid decarboxylase 67 was detected with immunohistochemistry. The co-localization of doublecortin with neuron-specific nuclear-binding protein in piriform cortex of adult guinea pigs was determined with immunofluorescence method. RESULTS AND CONCLUSION:The number of doublecortin, parvalbumin, glutamic acid decarboxylase 67 positive cel s in piriform cortex at the undeprived side was significantly higher than that at the deprived side at 3 and 6 weeks (P<0.05). There was no significant difference in the co-localization of doublecortin with neuron-specific nuclear-binding protein in piriform cortex at 3 weeks group and 6 weeks group (P>0.05). The findings suggest that olfactory experience promotes the maturation and differentiation of neurons in piriform cortex of adult guinea pigs.
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