An algal strain 18A8 (Micractinium sp.) isolated by our laboratory, is considered as a promising feedstock for biodiesel production. In this study, the algal strain was cultured on high-carbon medium with the addition of different concentrations of 2,4-D and GA3. Biomass and oil content were measured after ten days cultivation. The results showed that 2,4-D (2 μmol/L) and GA3 (1 μmol/L) could promote the growth of algae strain significantly. The corresponding max biomass was increased by 75.26% and 47.41% while the oil content decreased by 23.49% and 29.89% comparing to the control respectively; on the contrary, increasing the concentrations of 2,4-D (10 μmol/L) and GA3 (5 μmol/L) could obviously inhibit the growth of algae strain, the decrease in corresponding max biomass was 72.83% and 60.70% respectively. However, the oil content was increased by 149.45% and 95.75%. Moreover, the protein content, total sugar content, chlorophyll content and photosynthetic efficiency of the algae was decreased comparing to the control when 2,4-D (10 μmol/L) and GA3 (5 μmol/L) were added to thernmedium respectively.%实验室分离到1株含油量高并且生长迅速的微茫藻18A8 (Micractinium sp.)藻株,将其培养在高碳培养基TAP上,研究了不同浓度的植物激素2,4-二氯苯氧乙酸(2,4-D)和赤霉素(GA3)对其生物量和油脂积累的影响.结果表明:分别添加2,4-D(2 μmol/L)和GA3(1μmol/L)对藻株的生长有明显的促进作用,其最大生物量比对照组分别增加75.26％和47.41％,但其油脂含量与对照组相比却有所降低,分别降低了23.49％和29.89％;而当增加2,4-D和GA3浓度为10和5μmol/L时,对藻株的生长表现出明显的抑制作用,其最大生物量比对照组减少了72.83％和60.70％,但其油脂含量与对照组相比显著增加,分别增加149.45％和95.75％.同时,分别添加2,4-D(10 μmol/L)和GA3(5 μmol/L)的实验藻种的蛋白质和总糖含量较对照组有所降低,此外叶绿素含量和光合效率也降低.
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