目的：探讨苦参碱联合顺铂抑制人宫颈癌SiHa细胞及对肺癌肿瘤抑制因子1（TSLC1）基因表达的效果。方法取对数生长期的SiHa分为对照组、苦参组、顺铂组和联合组，采用四甲基偶氮唑蓝比色法（ MTT）测定4组联合干预SiHa细胞2d后的抑制率；采用实时荧光定量（ RT－PCR）技术检测各组处理后TSLC1基因mRNA表达水平的变化。结果对照组、顺铂组、苦参碱组和联合组的SiHa细胞抑制率分别为0、61．24％、70．31％和74．20％，药物干预组均高于对照组（ t 值分别为14．21、17．44、11．20，均P＜0．05），同时联合组高于苦参碱和顺铂单独干预（t值分别为4．47、3．21，均P＜0．05），而顺铂和苦参碱组间差异无统计学意义（t＝0．71，P＞0．05）。对照组、苦参碱组、顺铂组和联合组干预SiHa细胞后TSLC1mRNA表达量分别为1．00±0．00μg／mL，23．53±0．01μg／mL，84．33±0．03μg／mL和90．23±0．01μg／mL，苦参碱组、顺铂组和联合组干预组明显高于对照组（ z值分别为9．41、10．72、13．23，均P＜0．05），其中苦参碱组和联合组TSLC1mRNA表达量组间比较差异无统计学意义（z＝1．24，P＞0．05），但均高于顺铂组（z值分别为6．04、5．47，均P＜0．05）；析因设计方差分析结果表明顺铂和苦参碱均为SiHa细胞抑制率和TSLC1基因表达的影响因素，而且两者存在交互作用，联合应用使效果增加。结论苦参碱联合顺铂能抑制人宫颈癌SiHa细胞增殖，提高TSLC1mRNA表达量。%Objective To investigate the effect of matrine combined with cisplatin on inhibiting human cervical cancer SiHa cells and on tumor suppressor in lung cancer 1 (TSLC1) gene expression.Methods Logarithmic growth phase of SiHa cells were divided into control group, matrine group, cisplatin group and combination group.Inhibition rate was measured with MTT method.The change of TSLC1 gene mRNA expression was detected by RT-PCR process in each group.Results Inhibition rates of SiHa cells in the control group, matrine group, cisplatin group and the combination group were 0, 61.24%, 70.31%and 74.20%, respectively.The inhibition rates of medicine intervention groups were higher than the control group (t value was 14.21, 17.44 and 11.20, respectively, all P<0.05), and the combination group was higher than cisplatin and matrine group (t value was 4.47 and 3.21, respectively, both P<0.05).There was no significant difference between cisplatin group and matrine group (t=0.71, P>0.05).The levels of TSLC1 mRNA expression after the intervention on SiHa cells in the control group, matrine group, cisplatin group and the combination group were 1.00 ±0.00μg/mL, 23.53 ±0.01μg/mL, 84.33 ±0.03μg/mL and 90.23 ±0.01μg/mL, respectively.Those of the medicine intervention groups were significantly higher than the control group (z was 9.41, 10.72 and 13.23, respectively, all P<0.05).There was no significant difference between matrine group and the combination group in TSLC1 mRNA expression level (z=1.24,P>0.05), but both of them were higher than cisplatin group (z value was 6.04 and 5.47, respectively, both P<0.05).Factorial design analysis of variance showed that cisplatin and matrine were influencing factors of inhibiting SiHa cells and TSLC1 gene expression.There was an interaction effect between them so that it generated increasing effect by combination.Conclusion Matrine combined with cisplatin can inhibit the proliferation of human cervical cancer SiHa cells and improve TSLC1 mRNA expression level.
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