Objective To explore the correlation between detection of high-risk type human papilloma virus 16/18(HPVl6/18) DNA in pelvic lymph node in patients with cervical carcinoma and clinicopathologic features , and to investigate the correlation and clinical significance of primary cervical cancer focus and HPVDNA in pelvic lymph node .Methods With prospective study method , patients undergoing radical hysterectomy of cervical carcinoma in Ezhou Center Hospital from January 2011 to June 2012 were recruited .Polymerase chain reaction ( PCR) was adopted to test cervical cancer tissues of 33 cases and HPVl6/18 DNA in 115 pieces of pelvic lymph node . Results The positive detection rate of pelvic lymph node HPV16/18 in 33 cases was 54.55%(18/33), and it was correlated with clinical stage, lymph node metastasis , pathology differentiation degree , muscle layer infiltration depth , corpus and vaginal infiltration .The differences had statistical significance (χ2 value was 9.528, 5.241, 10.481, 7.187, 3.915 and 7.274, respectively, all P<0.05).The positive detection rate of HPV16/18 in cervical carcinoma tissue was 72.73%(24/33), and it was positively correlated with that of pelvic lymph node HPV16/18 (r=0.398, P<0.05).The positive detection rate of HPV16/18 of the total number in lymph node metastasis group and the group without lymph node metastasis was statistically different (χ2 =36.449, P<0.05).Conclusion Detecting pelvic lymph node is helpful to detect early occult metastases , and HPV can be used as specific marker of micro transfer .%目的：探讨宫颈癌患者盆腔淋巴结高危型人乳头状瘤病毒16/18（ HPVl6/18） DNA检出情况与宫颈癌临床病理特征的相关性，同时探讨宫颈癌原发灶和盆腔淋巴结组织中HPVDNA检出的相关性及意义。方法采用前瞻性研究方法，收集鄂州市中心医院自2011年1月至2012年6月间收治的行宫颈癌根治术患者的石蜡标本，采用聚合酶链反应（ PCR）方法，检测33例宫颈癌组织及115枚盆腔淋巴结组织中HPVl6/18 DNA。结果33例宫颈癌患者中盆腔淋巴结HPV16/18阳性检出率为54．55％（18/33），其阳性检出率与临床分期、有无淋巴结转移、病理分化程度、肌层浸润深度、有无宫体及阴道侵犯有关，差异均有统计学意义（χ2值分别为9．528、5．241、10．481、7．187、3．915、7．274，均P＜0．05）。宫颈癌组织HPV16/18阳性检出率为72．73％（24/33），与盆腔淋巴结HPV16/18阳性检出率进行相关分析，二者呈正相关（r＝0．398，P＜0．05）。淋巴结转移组与淋巴结未转移组总淋巴结个数的HPV16/18阳性检出率进行比较，差异有统计学意义（χ2＝36．449，P＜0．05）。结论检测盆腔淋巴结有助于发现早期隐匿性转移，HPV可以作为检测微转移的特异性标志物。
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