目的 研究人类白细胞抗原E(HLA-E)在反复自然流产(RSA)和正常流产人绒毛组织中的蛋白和mRNA表达,探讨HLA-E与不明原因RSA的相关性及其临床意义.方法 采用免疫组织化学技术和逆转录-聚合酶链反应技术检测35例RSA人绒毛组织和35例正常流产人绒毛组织中细胞学定位以及HLA-E在mRNA水平的表达.结果 免疫组织化学检测结果显示两组中均有HLA-E阳性表达,RSA组的HLA-E在人绒毛组织中阳性表达率低于正常流产组(χ2=131.125,P<0.05).逆转录-聚合酶链反应结果显示RSA组和正常流产组均有HLA-E表达,对比显示RSA组的HLA-E表达低于正常流产组(t=1.937,P<0.05).结论 RSA妊娠妇女早孕绒毛组织中HLA-E在组织细胞学蛋白和mRNA水平均较正常人工流产妊娠妇女早孕绒毛组织中降低,提示自然流产妊娠妇女存在HLA-E表达异常,HLA-E可能与RSA的发生关系密切.%Objective To investigate the correlation between human leukocyte antigen - E ( HLA-E ) and recurrent spontaneous abortion ( RSA ) as well as the significance of them through researching the expression of HLA-E in protein and mRNA level in human chorionic villi of women with RSA and with normal abortion. Methods Immunohistochemistry and reverse transcription-polymerase chain reaction technique were used to detect the localization of HLA-E and the expression of HLA-E in mRNA level in human chorionic villi of 35 women with RSA and 35 with normal abortion. Results The detection of immunohistochemistry showed that there were positive expressions of HLA-E in both groups, and that the expression of HLA-E in the group of women with RSA was lower than that in the group with normal abortion (χ2 = 131. 125,P <0.05 ). The results of the RT-PCR indicated expression of HLA-E in both groups and the expression in the group of women with RSA was lower than the group with normal abortion ( t = 1. 937 ,P < 0. 05 ). Conclusion The expression of HLA-E in protein and mRNA level in human chorionic villi of women with RSA is lower than that of women with normal abortion, which indicates that the expression of HLA-E is abnormal in women with RSA and that HLA-A may be closely related with the occurrence of RSA.
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