目的 检测炭疽芽胞杆菌中可变数量串联重复序列(VNTR)的变化,分析我国菌株中VNTR的特征和分布规律.方法 PCR扩增.琼脂糖电泳和毛细管电泳,测序验证,BLAST比对等.结果 11个VNTR位点中有4个在所有菌株中没有差别,另外7个位点只在个别菌株中检测到差别,有差别的菌株多分布在新疆；11个VNTR位点均位于基因编码区内,编码对细菌生存重要的蛋白和一些功能不明蛋白.结论 结果提示新疆的菌株类型较复杂；本研究中的VNTR位点比较保守,可能不能用于区别不同的炭疽芽胞杆菌,但对于了解炭疽芽胞杆菌的基因组特征以及鉴定炭疽芽胞杆菌具有一定的作用.%To study the characteristics and distribution of the variable number tandem repeat (VNTR) of Bacillus an-thracis in China, we selected 109 strains isolated from 17 provinces, and the polymorphism of 11 VNTR loci were studied by polymerase chain reaction (PCR). The PCR products were detected by agarose electrophoresis or capillary electrophoresis, and different DNA fragment in each VNTR locus were sequenced. The results showed no difference was found in 4 of 11 VNTR loci in tested strains; a few strains were detected different from most strains in 7 VNTR loci. All of VNTR loci were located in genes encoding important protein and some hypothetical protein. The results suggest that these strains isolated from Xinjiang were complicated. Although these VNTR loci varied too lightly to distinguish Bacillus anthracis strains, it's useful to understand the genome characteristics and identify Bacillus anthracis strains from other Bacillus species.
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