首页> 外国专利> SITE-SPECIFIC DNA MODIFICATION USING A DONOR DNA REPAIR TEMPLATE HAVING TANDEM REPEAT SEQUENCES

SITE-SPECIFIC DNA MODIFICATION USING A DONOR DNA REPAIR TEMPLATE HAVING TANDEM REPEAT SEQUENCES

机译:使用具有串联重复序列的配体DNA修复模板修饰特定位点的DNA

摘要

A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5′ flanking sequence and a donor 3′ flanking sequence. The donor 5′ flanking sequence and the donor 3′ flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.
机译:提供了一种对真核细胞的内源靶DNA进行位点特异性修饰的方法。该方法包括使具有预期修饰位点的内源靶DNA与(i)基因编辑系统接触,该基因编辑系统被配置为在预期修饰位点处或附近在内源靶DNA中引入双链断裂,和(ii)供体DNA修复模板包括多个串联重复序列。在该方法中,多个串联重复序列的每一个包含侧翼为供体5'侧翼序列和供体3'侧翼序列的外源供体DNA序列。供体5'侧翼序列和供体3'侧翼序列与内源靶DNA中预期修饰位点的任一侧上的连续DNA序列同源。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号