目的:研究五类化学指示卡和六类化学指示卡在高温压力蒸汽灭菌应用中的监测结果对比,以规范选择高压灭菌监测指示物.方法:将五类化学指示卡和六类化学指示卡同时放入高压灭菌锅4个角和中心位置进行灭菌,分别在常规灭菌参数134℃、240 s、220 kPa和模拟临界灭菌参数134℃、120 s、220 kPa下脉动3次进行灭菌试验.对比2种灭菌参数下,五类化学指示卡和六类化学指示卡监测的灭菌合格率.采用SPSS 19.0软件分析数据,差异比较用χ2检验.结果:在常规灭菌参数下,五类化学指示卡和六类化学指示卡监测灭菌合格率均为100%.在模拟临界灭菌参数下,五类化学指示卡监测灭菌合格率为100%,六类化学指示卡监测灭菌合格率为66.67%,差异有统计学意义(P<0.05).结论:相同条件下六类化学指示卡比五类化学指示卡具有更高的抗阻性.六类化学指示卡配合管腔挑战器使用更适用于灭菌参数不稳定的批量监测.%Objective To observe the effects of Class 5 and 6 chemical indicative cards used for monitoring pressure steam sterilization to standardize the procedure for selecting the monitoring substance of pressure steam sterilization. Methods Several pieces of Class 5 and 6 cards were placed at the four corners and center of the clave to undergo sterilization with the conventional parameters of 134 ℃, 240 s and 220 kPa as well as the simulation critical parameters of 134 ℃, 120 s and 220 kPa.The two kinds of cards had their qualification rates compared with two sets of parameters.SPSS 19.0 software was used for data analysis and Chi-square test was applied to comparison.Results The two kinds of cards had the qualification rates being 100% with the conventional parameters;with the simulation critical parameters,Class 5 card had the qualification rate being 100%,while Class 6 card had the rate of 66.67%,and there were significant difference between the qualification rates of the two cards(P<0.05).Conclusion Class 6 chemical indicative card has higher resistance and efficiency with the same conditions when compared with Class 5 card,which can be combined with the tube cavity challenger to execute mass monitoring with unstable parameters.
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